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The bacterial reverse mutation test uses amino acid-requiring strains of Salmonella typhimurium (S. typhimurium) and Escherichia coli (E. coli) to detect point mutations, which involve substitution, addition or deletion of one or a few DNA base pairs. The principle of this bacterial reverse mutation test is that it detects chemicals that induce mutations which revert mutations present in the tester strains and restore the functional capability of the bacteria to synthesize an essential amino acid. The revertant bacteria are detected by their ability to grow in the absence of the amino acid required by the parent tester strain.



Example

This is an example showing the report table, trial design, and results from a study's in vitro bacterial reverse mutation test using 4 different amino acid-requiring strains of S. typhimurium and 1 strain of E. coli to detect point mutations. 

This table shows the assay results for study 8325064, test article TA1, and 3 strains of salmonella (TA98, TA100, and TA1535) at varying concentrations. For brevity, the remaining tables (e.g., additional strains, samples prepared without metabolic activation) are not included. For ease of reference in the table below, each row has been labeled in the right-hand margin with the applicant-defined trial set label (e.g., SetA).  For brevity, only the four bolded sets are represented in the following example datasets.


This example Trial Summary (TS) dataset, shows many informational fields that provide context at the study level.   

Rows 1-2:

Show 2 records for TSPARMCD = "GLPTYP", using TSSEQ to indicate multiple records, since both GLP types apply for this study.

Row 3:Shows that this study was conducted as a GLP study.
Rows 4-5:Show the study start date and study title.
Rows 6-7:Show the version of SEND Implementation Guide and version of Controlled Terminology used in this study.
Row 8:Shows the applicant's organization.
Row 9:Shows that the applicant's study reference ID is not applicable.
Rows 10-13:Show that TSGRPID has been used to link records (name, location, country) related to the test facility (TSGRPID=1). The study director is associated with the test facility.
Rows 14-16:

Show that TSGRPID (TSGRPID=4) has been used to link the information on the testing guideline followed on this study (TSTGDNAM, TSTGDORG, TSTGDVER).

Row 17:Shows the study type for this study.
Row 18:Shows that this study includes a Bacterial Reverse Mutation Assay.
Rows 19-27:Show that TSGRPID (TSGRPID = 2) has been used to link the information for 1 species (salmonella) with the 4 different strains and cell lines that are tested in this study.
Rows 28-30:

Show that TSGRPID (TSGRPID = 3) has been used to link the information for 1 species (E. coli) with the strain and cell line that is tested in this study.

ts.xpt

xx.xpt

Row

STUDYID

DOMAIN

TSSEQ

TSGRPID

TSPARMCD

TSPARM

TSVAL

TSVALNF

18325064TS1
GLPTYPGood Laboratory Practice TypeFDA
28325064TS2
GLPTYPGood Laboratory Practice TypeOECD
38325064TS1
GLPFLGLP FlagN
48325064TS1
STSTDTCStudy Start Date2015-07-29
58325064TS1
STITLEStudy Title

The Bacterial Reverse Mutation Test, Study 8325064-1


68325064TS1
SNDIGVERSEND Implementation Guide VersionTOBACCO IMPLEMENTATION GUIDE VERSION 1.0
78325064TS1
SNDCTVERSEND Controlled Terminology VersionSEND Terminology 2021-09-30
88325064TS1
APPLCNTApplicantExample Applicant Inc.
98325064TS1
APREFIDApplicant's Study Reference ID
NOT APPLICABLE
108325064TS11TSTFNAMTest Facility NameExample Tox Lab Name
118325064TS11TSTFLOCTest Facility Location10 Somewhere Street, Montgomery, AL 10000
128325064TS11TFCNTRYTest Facility CountryUSA
138325064TS11STDIRStudy DirectorDr. R. Smith
148325064TS14TSTGDNAMTesting Guideline NameTest NO. 471
158325064TS14TSTGDORGTesting Guideline OrganizationOECD
168325064TS14TSTGDVERTesting Guideline Version2020-06-29
178325064TS1
SSTYPStudy TypeGENOTOXICITY IN VITRO
188325064TS1

GNTXAID

Genetic Toxicology Assay Identifier

AMES


198325064TS12

SPECIES

Species

SALMONELLA TYPHIMURIUM


208325064TS12STRAINStrain/SubstrainTA98
218325064TS22STRAINStrain/SubstrainTA100
228325064TS32STRAINStrain/SubstrainTA1535
238325064TS42STRAINStrain/SubstrainTA1537
248325064TS12CELLLNCell LineTA 98 hisD3052; rfa-; uvrB-
258325064TS22CELLLNCell LineTA 100 hisG46; rfa-; uvrB-
268325064TS32CELLLNCell LineTA 1535 hisG46; rfa-; uvrB-
278325064TS42CELLLNCell LineTA 1537 hisC3076; rfa-; uvrB-
288325064TS23SPECIESSpecies

ESCHERICHIA COLI


298325064TS53STRAINStrain/SubstrainWP2 uvrA pKM101
308325064TS53CELLLNCell LinetrpE uvrA
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This example Trial Set (TX) dataset shows information about the test conditions for SetA and SetR in this study. For brevity, the dataset does not show information for SetF, SetG, or any other sets. A fully formed TX dataset for this example study would include information about the test conditions for all sets. 

Note that there are three trial set parameters that link to other important datasets: SPTOBID, APDEVID, and SMKRGM. 

  • SPTOBID (Applicant-Defined Tobacco Product ID) is used to uniquely identify the tobacco product. The value of SPTOBID (e.g., CIG01a) matches the value for SPTOBID in all the TOPARMCD-TOVAL pairs in the Tobacco Product Identifiers and Descriptors (TO) dataset example in Section 3.1.2, Product Design Parameters and Conformance Testing, Example 1. The TOPARMCD-TOVAL pairs identify this unique product, CIG01a.  The TO domain is described in Section 2.8.8.1, Tobacco Product Identifiers and Descriptors (TO).
  • The value of APDEVID (e.g., PUFFMASTER3K) matches the value of SPDEVID in all the DIPARMCD-DIVAL pairs that identify this unique device in the DI dataset. SPDEVID is the applicant-defined device identifier that is used to uniquely identify the device in the Unique Device Identification (DI) dataset (see also Section 2.8.9.7, SEND Device Identifiers (DI)). This is shown in the DI dataset in Section 3.1.3.2, HPHCs, Other Constituents, and Smoking/Vaping Regimens, Example 1.
  • SMKRGM serves as a link to the Device-In Use Properties (DU) domain (see also Section 2.8.9.8, SEND Device-In-Use (DU)), where a matching value of SMKRGM indicates parameters of the smoking regimen performed by the smoking machine, as in the DU dataset in Section 3.1.3.2, HPHCs, Other Constituents, and Smoking/Vaping Regimens, Example 2.

Rows 1-22:

Show trial set parameters and trial set values that are the test conditions specific to set SetA which is the vehicle control (i.e., Strain of TA98, vehicle control with concentration value of 0). SetA is associated with the first row, labeled "SetA", in the report table for example study 8325064.

Rows 23-44:

Show trial set parameters and trial set values that comprise the test conditions for the set SetF which is the strain of TA98 with a concentration value of 500 µg/plate. SetF is associated with the sixth row, labeled "SetF", in the report table for example study 8325064.

Rows 45-66:Show trial set parameters and trial set values that comprise the test conditions for the set SetG which is the strain of TA98 with a concentration value of 1600 µg/plate. SetG is associated with the seventh row, labeled "SetG", in the report table for example study 8325064.
Rows 67-88:Show trial set parameters and trial set values that comprise the test conditions for the set SetR which is the strain of TA100, positive control with a concentration value of 2 µg/plate. SetR is associated with the eighteenth row, labeled "SetR", in the report table for example study 8325064.

tx.xpt

tx.xpt

RowSTUDYIDDOMAINSETCDSETTXSEQTXPARMCDTXPARMTXVAL
18325064TXSetAA-TA98-C01SPECIESSpeciesSALMONELLA TYPHIMURIUM
28325064TXSetAA-TA98-C02IVTDMINIn vitro Treatment Duration Minimum71.5
38325064TX

SetA

A-TA98-C03IVTDTRGIn vitro Treatment Duration Target72
48325064TX

SetA

A-TA98-C04IVTDMAXIn vitro Treatment Duration Maximum72.5
58325064TXSetAA-TA98-C05IVTDUIn vitro Treatment Duration UnitHOURS
68325064TXSetAA-TA98-C06INCBTMPIncubation Temperature37
78325064TXSetAA-TA98-C07INCBTMPUIncubation Temperature UnitC
88325064TXSetAA-TA98-C08ATMRHPAtmospheric Relative Humidity Percent50
98325064TXSetAA-TA98-C09ATMCO2PAtmospheric CO2 Percent5
108325064TXSetAA-TA98-C010SPTOBID

Applicant-defined tobacco identifier

CIG01a
118325064TXSetAA-TA98-C011EXPTYP

Exposure Type

Submerged
128325064TX

SetA

A-TA98-C012SAMTYP

Sample Type

Total Particulate Matter in PBS
138325064TX

SetA

A-TA98-C013APDEVIDApplicant-defined device identifierPUFFMASTER3K
148325064TXSetAA-TA98-C014SMKRGMSmoking RegimenNON-INTENSE REGIMEN
158325064TXSetAA-TA98-C015STRAINStrain/SubstrainTA98
168325064TXSetAA-TA98-C016MTACTINDMetabolic Activating Agent NameNOT APPLICABLE
178325064TXSetAA-TA98-C017METACTFLPresence of Metabolic Activation FlagN
188325064TXSetAA-TA98-C018ITVNAM

Intervention Article Name

DMSO
198325064TXSetAA-TA98-C019ITVTYPE

Intervention Article Type

VEHICLE
208325064TXSetAA-TA98-C020ITVCONCIntervention Article Concentration100
218325064TXSetAA-TA98-C021ITVCONCUIntervention Article Concentration Unit%
228325064TXSetAA-TA98-C022TRTVTreatment VehicleDMSO
238325064TXSetFF-TA98-C50023SPECIESSpeciesSALMONELLA TYPHIMURIUM
248325064TXSetFF-TA98-C50024IVTDMINIn vitro Treatment Duration Minimum71.5
258325064TXSetFF-TA98-C50025IVTDTRGIn vitro Treatment Duration Target72
268325064TXSetFF-TA98-C50026IVTDMAXIn vitro Treatment Duration Maximum72.5
278325064TXSetFF-TA98-C50027IVTDUIn vitro Treatment Duration UnitHOURS
288325064TXSetFF-TA98-C50028INCBTMPIncubation Temperature37
298325064TXSetFF-TA98-C50029INCBTMPUIncubation Temperature UnitC
308325064TXSetFF-TA98-C50030ATMRHPAtmospheric Relative Humidity Percent50
318325064TXSetFF-TA98-C50031ATMCO2PAtmospheric CO2 Percent5
328325064TXSetFF-TA98-C50032SPTOBID

Applicant-defined tobacco identifier

CIG01a
338325064TXSetFF-TA98-C50033EXPTYP

Exposure Type

Submerged
348325064TXSetFF-TA98-C50034SAMTYP

Sample Type

Total Particulate Matter in PBS
358325064TXSetFF-TA98-C50035APDEVIDApplicant-defined device identifierPUFFMASTER3K
368325064TXSetFF-TA98-C50036SMKRGMSmoking RegimenNON-INTENSE REGIMEN
378325064TXSetFF-TA98-C50037STRAINStrain/SubstrainTA98
388325064TXSetFF-TA98-C50038MTACTINDMetabolic Activating Agent NameNOT APPLICABLE
398325064TXSetFF-TA98-C50039METACTFLPresence of Metabolic Activation FlagN
408325064TXSetFF-TA98-C50040ITVNAM

Intervention Article Name

TA1
418325064TXSetFF-TA98-C50041ITVTYPE

Intervention Article Type

PRODUCT
428325064TXSetFF-TA98-C50042ITVCONCIntervention Article Concentration500
438325064TXSetFF-TA98-C50043ITVCONCUIntervention Article Concentration Unitug/plate
448325064TXSetFF-TA98-C50044TRTVTreatment VehicleDMSO
458325064TXSetGG-TA98-C160045SPECIESSpeciesSALMONELLA TYPHIMURIUM
468325064TXSetGG-TA98-C160046IVTDMINIn vitro Treatment Duration Minimum71.5
478325064TXSetGG-TA98-C160047IVTDTRGIn vitro Treatment Duration Target72
488325064TXSetGG-TA98-C160048IVTDMAXIn vitro Treatment Duration Maximum72.5
498325064TXSetGG-TA98-C160049IVTDUIn vitro Treatment Duration UnitHOURS
508325064TXSetGG-TA98-C160050INCBTMPIncubation Temperature37
518325064TXSetGG-TA98-C160051INCBTMPUIncubation Temperature UnitC
528325064TXSetGG-TA98-C160052ATMRHPAtmospheric Relative Humidity Percent50
538325064TXSetGG-TA98-C160053ATMCO2PAtmospheric CO2 Percent5
548325064TXSetGG-TA98-C160054SPTOBID

Applicant-defined tobacco identifier

CIG01a
558325064TXSetGG-TA98-C160055EXPTYP

Exposure Type

Submerged
568325064TXSetGG-TA98-C160056SAMTYP

Sample Type

Total Particulate Matter in PBS
578325064TXSetGG-TA98-C160057APDEVIDApplicant-defined device identifierPUFFMASTER3K
588325064TXSetGG-TA98-C160058SMKRGMSmoking RegimenNON-INTENSE REGIMEN
598325064TXSetGG-TA98-C160059STRAINStrain/SubstrainTA98
608325064TXSetGG-TA98-C160060MTACTINDMetabolic Activating Agent NameNOT APPLICABLE
618325064TXSetGG-TA98-C160061METACTFLPresence of Metabolic Activation FlagN
628325064TXSetGG-TA98-C160062ITVNAM

Intervention Article Name

TA1
638325064TXSetGG-TA98-C160063ITVTYPE

Intervention Article Type

Product
648325064TXSetGG-TA98-C160064ITVCONCIntervention Article Concentration1600
658325064TXSetGG-TA98-C160065ITVCONCUIntervention Article Concentration Unitug/plate
668325064TXSetGG-TA98-C160066TRTVTreatment VehicleDMSO
678325064TX

SetR

R-TA100-C267SPECIESSpeciesSALMONELLA TYPHIMURIUM
688325064TX

SetR

R-TA100-C268IVTDMINIn vitro Treatment Duration Minimum71.5
698325064TX

SetR

R-TA100-C269IVTDTRGIn vitro Treatment Duration Target72
708325064TX

SetR

R-TA100-C270IVTDMAXIn vitro Treatment Duration Maximum72.5
718325064TX

SetR

R-TA100-C271IVTDUIn vitro Treatment Duration UnitHOURS
728325064TX

SetR

R-TA100-C272INCBTMPIncubation Temperature37
738325064TX

SetR

R-TA100-C273INCBTMPUIncubation Temperature UnitC
748325064TX

SetR

R-TA100-C274ATMRHPAtmospheric Relative Humidity Percent50
758325064TX

SetR

R-TA100-C275ATMCO2PAtmospheric CO2 Percent5
768325064TX

SetR

R-TA100-C276SPTOBID

Applicant-defined tobacco identifier

CIG01a
778325064TX

SetR

R-TA100-C277EXPTYP

Exposure Type

Submerged
788325064TX

SetR

R-TA100-C278SAMTYP

Sample Type

Total Particulate Matter in PBS
798325064TX

SetR

R-TA100-C279APDEVIDApplicant-defined device identifierPUFFMASTER3K
808325064TX

SetR

R-TA100-C280SMKRGMSmoking RegimenNON-INTENSE REGIMEN
818325064TX

SetR

R-TA100-C281STRAINStrain/SubstrainTA100
828325064TX

SetR

R-TA100-C282MTACTINDMetabolic Activating Agent NameNOT APPLICABLE
838325064TX

SetR

R-TA100-C283METACTFLPresence of Metabolic Activation FlagN
848325064TX

SetR

R-TA100-C284ITVNAM

Intervention Article Name

NaN3
858325064TX

SetR

R-TA100-C285ITVTYPE

Intervention Article Type

Positive Control
868325064TX

SetR

R-TA100-C286ITVCONCIntervention Article Concentration2
878325064TX

SetR

R-TA100-C287ITVCONCUIntervention Article Concentration Unitug/plate
888325064TX

SetR

R-TA100-C288TRTVTreatment VehicleDMSO
$warningHtml

Dataset Wrapper Debug Message

Please add a row column to your dataset.

REFID values are defined by the applicant to uniquely identify the observational unit within an experimental unit. In the simplified diagram of an assay procedure (below), the test tube that contains the possible mutagen is an example of an entity that would have a REFID indicating information at the level of trial set. The petri plate created from that test tube and used to count revertant numbers is an example of an entity that would be at an observational level. 

This picture does not depict the more complicated procedures, trial sets, or observational units for the example study. For study 8325064, the applicant chose to define the REFIDs at the trial set level with a single character (e.g., A, F, G, R) and chose to define REFIDs at the observational level based on the location of each tube in 1 of multiple exposure/incubation plates (e.g, 6_1 for plate 6, position 1). 


Row 1:

Shows the value of REFID=A. This REFID refers to the trial set with a SETCD of "SetA", as defined in the TX dataset. LEVEL=1 and LVLDESC="EXPERIMENTAL UNIT/TRIAL SET". TRIAL SET indicates this identifier relates to the entire trial set. EXPERIMENTAL UNIT indicates this identifier received the intervention article, DMSO.

Rows 2-6:Show the values of 5 observational units (1_1 through 1_5) that are within the parent experimental unit, REFID=A.
Row 7:Shows the value of REFID=F. This REFID refers to the trial set with a SETCD of "SetF", as defined in the TX dataset. LEVEL=1 and LVLDESC="EXPERIMENTAL UNIT/TRIAL SET". TRIAL SET indicates this identifier relates to the entire trial set. EXPERIMENTAL UNIT indicates this identifier received the intervention article, TA1.
Rows 8-10:Show the values of 3 observational units (6_1 through 6_3) that are within the parent experimental unit, REFID=F.
Row 11:Shows the value of REFID=G. This REFID refers to the trial set with a SETCD of "SetG", as defined in the TX dataset. LEVEL=1 and LVLDESC="EXPERIMENTAL UNIT/TRIAL SET". TRIAL SET indicates this identifier relates to the entire trial set. EXPERIMENTAL UNIT indicates this identifier received the intervention article, TA1.
Rows 12-14:Show the values of 3 observational units (7_1 through 7_3) that are within the parent experimental unit, REFID=G.
Row 15:Shows the value of REFID=R. This REFID refers to the trial set with a SETCD of "SetG", as defined in the TX dataset. LEVEL=1 and LVLDESC="EXPERIMENTAL UNIT/TRIAL SET". TRIAL SET indicates this identifier relates to the entire trial set. EXPERIMENTAL UNIT indicates this identifier received the intervention article, NaN3.
Rows 16-18:

Show the values of 3 observational units (18_1 through 18_3) that are within the parent experimental unit, REFID=R.

$titleHtml

relref.xpt

Row

STUDYID

SETCD

REFID

PARENT

LEVEL

LVLDESC

1

8325064

SetA

A


1

EXPERIMENTAL UNIT/TRIAL SET

2

8325064

SetA

1_1

A

2

OBSERVATIONAL UNIT

3

8325064

SetA

1_2

A

2

OBSERVATIONAL UNIT

4

8325064

SetA

1_3

A

2

OBSERVATIONAL UNIT

5

8325064

SetA

1_4

A

2

OBSERVATIONAL UNIT

6

8325064

SetA

1_5

A

2

OBSERVATIONAL UNIT

7

8325064

SetF

F


1

EXPERIMENTAL UNIT/TRIAL SET

8

8325064

SetF6_1F

2

OBSERVATIONAL UNIT

9

8325064

SetF6_2F

2

OBSERVATIONAL UNIT

10

8325064

SetF

6_3F

2

OBSERVATIONAL UNIT

11

8325064

SetG

G


1

EXPERIMENTAL UNIT/TRIAL SET

12

8325064

SetG7_1G

2

OBSERVATIONAL UNIT

13

8325064

SetG

7_2G

2

OBSERVATIONAL UNIT

14

8325064

SetG7_3G

2

OBSERVATIONAL UNIT

15

8325064

SetR

R


1

EXPERIMENTAL UNIT/TRIAL SET

16

8325064

SetR18_1R

2

OBSERVATIONAL UNIT

17

8325064

SetR18_2R

2

OBSERVATIONAL UNIT

18

8325064

SetR18_3R

2

OBSERVATIONAL UNIT

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Dataset Wrapper Debug Message

Please add a row column to your dataset.

Rows 1-5:Show the number of revertant colonies per plate collected for each of 5 observational units, GTREFID=1_1 through 1_5 (see description in the RELREF dataset).
Rows 6, 7:Show summary values collected (mean, standard deviation) for GTREFID=A that apply to the entire trial set, SetA, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID as shown in the RELREF dataset.
Rows 8-13:Revertent colonies were counted for each of 3 plates/observational units (GTREFID=6_1 through 6_3) and each value is associated with a record to show a postfix code of "V" (Very thin background bacterial lawn).
Rows 14-16:Show summary values collected (mean, standard deviation, fold increase) for GTREFID=F that apply to the entire trial set, SetF, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID in the RELREF dataset.
Rows 17-19:

Show 3 plates/observational units (GTREFID=7_1 through 7_3) where no revertant colonies were counted due to too much cytotoxicity and a postfix code of "T". TOBA-668 - Jira project doesn't exist or you don't have permission to view it.

Rows 20-22:Show the number of revertant colonies per plate collected for each of 3 observational units (GTREFID=18_1 through 18_3).
Rows 23-25:Show summary values collected (mean, standard deviation, fold increase) for GTREFID=R that apply to the entire trial set, SetR, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID in the RELREF dataset.

gt.xpt

gt.xpt

RowSTUDYIDDOMAINGTSEQGTREFIDGTTESTCDGTTESTGTORRESGTORRESUGTCOLSRTGTSTRESCGTSTRESNGTSTRESUGTSTATGTREASNDGTMETHODGTDTC
18325064GT11_1RPPRevertant Colony Numbers Per Plate26

2626




INSTRUMENT COUNTED

2015-08-03
28325064GT21_2RPPRevertant Colony Numbers Per Plate35

3535


INSTRUMENT COUNTED2015-08-03
38325064GT31_3RPPRevertant Colony Numbers Per Plate39

3939


INSTRUMENT COUNTED2015-08-03
48325064GT41_4RPPRevertant Colony Numbers Per Plate35

3535


INSTRUMENT COUNTED

2015-08-03
58325064GT51_5RPPRevertant Colony Numbers Per Plate30

3030


INSTRUMENT COUNTED2015-08-03
68325064GT6ARPPRevertant Colony Numbers Per Plate33.0
MEAN33.033.0



2015-08-03
78325064GT7ARPPRevertant Colony Numbers Per Plate5.0
STANDARD DEVIATION5.05.0



2015-08-03
88325064GT86_1RPPRevertant Colony Numbers Per Plate8

88


MANUALLY COUNTED2015-08-03
98325064GT96_1CYTOTOXCytotoxicityVery thin background bacterial lawn

V




2015-08-03
108325064GT106_2RPPRevertant Colony Numbers Per Plate10

1010


MANUALLY COUNTED2015-08-03
118325064GT116_2CYTOTOXCytotoxicityVery thin background bacterial lawn

V




2015-08-03
128325064GT126_3RPPRevertant Colony Numbers Per Plate12

1212


MANUALLY COUNTED2015-08-03
138325064GT136_3CYTOTOXCytotoxicityVery thin background bacterial lawn

V




2015-08-03
148325064GT14FRPPMEANMean Rev Colony Num Per Plate

10.0


MEAN

10.0

10.0





2015-08-03
158325064GT15FRPPSTDDVStd Dev Rev Colony Num Per Plate2.0
STANDARD DEVIATION2.02.0



2015-08-03
168325064GT16FRPPFLDICFold Increase Rev Colony Num Per Plate0.3
FOLD INCREASE0.30.3



2015-08-03
178325064GT177_1CYTOTOXCytotoxicity

Toxic No Revertant Colonies



T

NOT DONETOO MUCH CYTOTOXICITY
2015-08-03
188325064GT187_2CYTOTOXCytotoxicityToxic No Revertant Colonies

T

NOT DONETOO MUCH CYTOTOXICITY
2015-08-03
198325064GT197_3CYTOTOXCytotoxicityToxic No Revertant Colonies

T

NOT DONETOO MUCH CYTOTOXICITY
2015-08-03
208325064GT2018_1RPPRevertant Colony Numbers Per Plate

812



812

812




INSTRUMENT COUNTED

2015-08-03
218325064GT2118_2RPPRevertant Colony Numbers Per Plate

912



912

912




INSTRUMENT COUNTED2015-08-03
228325064GT2218_3RPPRevertant Colony Numbers Per Plate

839



839

839




INSTRUMENT COUNTED2015-08-03
238325064GT23RRPPMEANMean Rev Colony Num Per Plate854.3
MEAN854.3854.3



2015-08-03
248325064GT24RRPPSTDDVStd Dev Rev Colony Num Per Plate51.7
STANDARD DEVIATION51.751.7



2015-08-03
258325064GT25RRPPFLDICFold Increase Rev Colony Num Per Plate6.4
FOLD INCREASE6.46.4



2015-08-03
$warningHtml

Dataset Wrapper Debug Message

Please add a row column to your dataset.

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