The lab terminology team has been working on trying to determine the best way of representing the Flow Cytometry data in the lab test codelist. In the past, there have been multiple team meetings and a large discussion at an Intrachange. We have been recently discussing a new approach for how to handle this data.
Test Code = <8 character short name>
Test Name = <Core Cell Type>
Definition = A measurement of the <core cell type> in a biological specimen.
Craig - Immunophenotyping Working CMAP Image.jpg
Craig Zwickl created the following table:
Cell Name (Test Name) | Species | Operational Definition (criteria used to classify) | Common Name | Sponsor's Name | Sponsor's Cell Name | Species | Sponsor's Receptor Pattern | Sponsor's Result Expression | Sponsor's Result Unit(s) |
|---|---|---|---|---|---|---|---|---|---|
| T Cell (pan) | CD3+ | CD3 | Janssen | Human | |||||
| T Cell (DN1) | CD4-CD8-CD44+CD25- ; CD4-CD8-CD44+CD25- | ||||||||
| T Cell (DN2) | CD4-CD8-CD44+CD25+ ; CD3+CD4-CD8-CD44+CD25+ | ||||||||
| T Cell (DN3) | CD4-CD8-CD44-CD25+ ; CD3+CD4-CD8-CD44-CD25+ | ||||||||
| T Cell (DN4) | CD4-CD8-CD44-CD25- ; CD3+CD4-CD8-CD44-CD25- | ||||||||
| T Cell (DP) | CD4+CD8+ ; CD3+CD4+CD8+ | ||||||||
| T Cell (CD4P) | CD4+ ; CD3+CD4+ ; CD4+CD8- ; CD3+CD4+CD8- | CD3+CD4+CD25+CD127dim | Janssen | Human | CD25+CD127dim
| ||||
| T Cell (CD8P) | CD8+ ; CD3+CD8+ ; CD4-CD8+ ; CD3+CD4-CD8+ | Cytotoxic T Cell | |||||||
| T Cell (Th1) | CD4+CXCR3+ ; CD3+CD4+CXCR3+ ; CD4+CD8-CXCR3+ ; CD3+CD4+CD8-CXCR3+ ; CD4+CD8-CCR6-CXCR3+ ; CD3+CD4+CD8-CCR6-CXCR3+ ; CD4+CD8-CD196-CXCR3+ ; CD3+CD4+DC8-CD196-CXCR3+ | T Helper 1 | |||||||
| T Cell (Th2) | CD4+CCR4+ ; CD4+CCR4+Crth2+ ; CD3+CD4+CCR4+ ; CD3+CD4+CCR4+Crth2+ ; CD4+CD8-CCR4+ ; CD4+CD8-CCR4+Crth2+ ; CD3+CD4+CD8-CCR4+ ; CD3+CD4+CD8-CCR4+Crth2+ CD4+CD8-CCR6-CXCR3- ; CD4+CD8-CD196-CXCR3- ; CD3+CD4+CD8-CCR6-CXCR3- ; CD3+CD4+CD8-CD196-CXCR3- | T Helper 2 | |||||||
| T Cell (Th9) | CD4+ ; | ||||||||
| T Cell (Th17) | CD4+CCR6+ ; CD4+CD196+ ; CD3+CD4+CCR6+ ; CD3+CD4+CD196+ ; CD4+CD8-CCR6+ ; CD4+CD8-CD196+ ; CD3+CD4+CD8-CCR6+ ; CD3+CD4+CD8-CD196+ ; CD4+CD8-CCR6+CXCR3- ; CD3+CD4+CD8-CCR6+CXCR3- ; CD4+CD8-CD196+CXCR3- ; CD3+CD4+CD8-CD196+CXCR3- | ||||||||
| T Cell (Tfh) | CD4+CXCR5+ | ||||||||
| T Cell (Th22) | |||||||||
| T Cell (nTreg) | CD4+CD25+FoxP3+ ; CD4+CD25(HI)FoxP3+ | Natural T Regulatory Cell | |||||||
| Natural Killer T Cell (NKT) | |||||||||
Cell Name (Test Name) | Species | Operational Definition (criteria used to classify) | Common Name | Sponsor's Name | Sponsor's Cell Name | Species | Sponsor's Receptor Pattern | Sponsor's Result Expression | Sponsor's Result Unit(s) |
|---|---|---|---|---|---|---|---|---|---|
| T Cell | CD4+CD25(HI)CD127-CD45RA- | ||||||||
| T Cell (nTreg), expandable | CD4+CD25+CD127- | ||||||||
| T Cell (iTreg) | Inducible T Regulatory Cell | ||||||||
| Other Phenotypic Markers: | |||||||||
| HLA-DR (activation) | |||||||||
| V500 (viability dye) | |||||||||
| CD45RO (memory) | |||||||||
| CD38 (activation) | |||||||||
| CD27 (memory) | |||||||||
| T Cell (CD4P), Effector Memory | CD3+CD4+CD8-CD45RO+CCR7- | ||||||||
| T Cell (CD4P), Effector | CD3+CD4+CD8-CD45RO-CCR7- | ||||||||
| T Cell (CD4P), Central Memory | CD3+CD4+CD8-CD45RO+CCR7+ | ||||||||
| T Cell (CD4P), Naïve | CD3+CD4+CD8-CD45RO-CCR7+ | ||||||||
| T Cell (CD4P), Activated APC | CD3+CD4+CD8-(CD27+CD127+)CD38+HLA/DR+ |
Cell Name (Test Name) | Species | Operational Definition (criteria used to classify) | Common Name | Sponsor's Name | Sponsor's Cell Name | Species | Sponsor's Receptor Pattern | Sponsor's Result Expression | Sponsor's Result Unit(s) |
|---|---|---|---|---|---|---|---|---|---|
| T Cell (CD4P), Treg | CD4+CD8-CD25+CD127(DIM) | Amgen | T_4+Treg | CD25+CD127+dim||CD3+CD4+||CD45+Lymph | % EVENT COUNT | ||||
| T Cell (CD4P), Treg | Amgen | T_4+Treg ICOS+ | ICOS+||CD25+CD127+dim||CD3+CD4+||CD45+Lymph | % EVENT COUNT MESF | |||||
| T Cell (CD4P), Treg, Memory | CD4+CD8-CD25+CD127(DIM)CD45RO+HLA/DR- | ||||||||
| T Cell (CD4P), Treg, Naïve | CD4+CD8-CD25+CD127(DIM)CD45RO-HLA/DR- | Amgen | T_4+nonTreg | CD25-CD127+||CD3+CD4+||CD45+Lymph | % EVENT COUNT | ||||
| T Cell (CD4P), Treg, Naïve | Amgen | T_4+nonTreg | ICOS+||CD25-CD127+||CD3+CD4+||CD45+Lymph | % EVENT COUNT MESF | |||||
| T Cell (CD4P), Treg, Memory, Activated | CD4+CD8-CD25+CD127(DIM)CD45RO+HLA/DR- | ||||||||
| T Cell (CD8P) | |||||||||
Cell Name (Test Name) | Species | Operational Definition (criteria used to classify) | Common Name | Sponsor's Name | Sponsor's Cell Name | Species | Sponsor's Receptor Pattern | Sponsor's Result Expression | Sponsor's Result Unit(s) |
|---|---|---|---|---|---|---|---|---|---|
| Mouse B Cells: | |||||||||
| B Cell (pan) | B220+ ; CD45R+ ; CD45R/B220+ | ||||||||
| B Cell (PreProB) | B220(lo)BP1-CD24(lo) ; CD45R(lo)BP1-CD24(-/lo) ; B220+CD43(lo)CD24(-/lo) | ||||||||
| B Cell (PreB) | B220(lo)BP1+CD24+ ; CD45R(lo)BP1+CD24+ | ||||||||
| B Cell, Developing | B220+CD43-CD24+ | ||||||||
| B Cell (ProB, PreB) | B220+CD43-CD24+sIgM-sIgD- | ||||||||
| B Cell, Immature | B220+CD43-CD24+sIgM(lo)sIgD- | ||||||||
| B Cell, Transitional | B220+CD43-CD24+sIgM+sIgD- | ||||||||
| B Cell, Early Mature | B220+CD43-CD24+sIgM+sIgD+ | ||||||||
| B Cell, Late Mature | B220+CD43-CD24+sIgM(lo)sIgD+ | ||||||||
Cell Name (Test Name) | Species | Operational Definition (criteria used to classify) | Common Name | Sponsor's Name | Sponsor's Cell Name | Species | Sponsor's Receptor Pattern | Sponsor's Result Expression | Sponsor's Result Unit(s) |
|---|---|---|---|---|---|---|---|---|---|
| Human B Cells: | |||||||||
| B Cell (pan) | CD19+ ; CD3-CD19+ | ||||||||
| B Cell, CD20- | CD19+CD20- ; CD3-CD19+CD20- | ||||||||
| B Cell, CD20+ | CD19+CD20+ ; CD3-CD19+CD20+ | ||||||||
| B Cell, Memory | CD19+CD27+sIgD- | ||||||||
| B Cell, Memory, Non-Class-Switched | CD19+CD27+sIgD+ | ||||||||
| B Cell, Memory, Class-Switched | CD19+CD27+sIgD-sIgM-CD20+CD38+/- | ||||||||
| B Cell, Naïve | CD19+CD27-sIgD+ | ||||||||
| B Cell, Transitional | CD19+CD24++CD38++ | ||||||||
| B Cell, Plasmablast | CD19+CD24-CD38- ; CD19+CD27+sIgD-sIgM-CD20-CD38++ | ||||||||
| B Cell, Plasma Cell | CD19+CD138+CD38+ ; CD138+CD38+ | ||||||||
| The above can also be differentiated by simultaneous staining with: | CD19, CD20, sIgD, CD27, CD38, CD24, CD3, CD138, sIgM, sIgG | ||||||||
| B Cell, Effector 1 (Be-1) | |||||||||
| B Cell, Effector 2 (Be-2) | |||||||||
| B Cell, Regulatory | |||||||||
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