SEND Example.In vitro Bacterial Reverse Mutation Assay

This is an example showing the report table, trial design, and results from the in vitro bacterial reverse mutation test of an example study, Study #8325064. This bacterial reverse mutation test uses four different amino acid-requiring strains of Salmonella typhimurium (S. typhimurium) and one strain of Escherichia coli (E. coli) to detect point mutations, which involve substitution, addition or deletion of one or a few DNA base pairs. TOBA-129 - Getting issue details... STATUS

In this example, the Trial Summary dataset, ts.xpt, includes many informational fields that may provide context at the study level, for Study 8325064. Also, TSGRPID has been used to link records (name, location, country) related to the test facility (TSGRPID = 1) and records related to the Test Site (TSGRPID = 2). The Study Director is associated with the Test Facility and the Principal Investigator is associated with the Test Site. Finally, the Primary Treatment CAS Registry Number is not known and this is recorded as an empty TSVAL and UNKNOWN in the corresponding TSVALNF.

This table shows the assay results for Study 8325064, Test Article TA1, and three strains of Salmonella (TA98, TA100, and TA1535) at varying concentrations. For ease of reference, each row has been labeled in the right hand margin with the sponsor defined trial set label (e.g., SetA). For brevity, the remaining tables (e.g., additional strains, samples prepared without metabolic activation, etc.) are not included.

Rows 1-2:	Show two records for TSPARMCD = "GLPTYP", using TSSEQ to indicate multiple records, since both GLP Types apply for this example study.
Row 8:	Shows that the sponsor's study reference ID is not applicable.
Rows 9-12:	Show that TSGRPID has been used to link records (name, location, country) related to the test facility (TSGRPID = 1). The Study Director is associated with the Test Facility.
Rows 18-24:	Show that this example study includes two different species of bacteria and a total of five strains.
Rows 18-22:	Show that TSGRPID (TSGRPID = 2) has been used to link the information for one species (Salmonella) with four different strains that are tested in this example study.
Rows 23-24:	Show that TSGRPID (TSGRPID = 3) has been used to link the information for one species (Escherichia coli) with the strain that is tested in this example study.

ts.xpt

xx.xpt

Row	STUDYID	GNTXAID	DOMAIN	TSSEQ	TSGRPID	TSPARMCD	TSPARM	TSVAL	TSVALNF
1	8325064	Ames	TS	1		GLPTYP	Good Laboratory Practice Type	FDA
2	8325064	Ames	TS	2		GLPTYP	Good Laboratory Practice Type	OECD
3	8325064	Ames	TS	1		STSTDTC	Study Start Date	2015-07-29
4	8325064	Ames	TS	1		STITLE	Study Title	The Bacterial Reverse Mutation Test, Study 8325064-1
5	8325064	Ames	TS	1		SNDIGVER	SEND Implementation Guide Version	TOBACCO IMPLEMENTATION GUIDE VERSION 1.0
6	8325064	Ames	TS	1		SNDCTVER	SEND Controlled Terminology Version	SEND Terminology 2021-09-30
7	8325064	Ames	TS	1		SSPONSOR	Sponsor Organization	Example Sponsor Inc.
8	8325064	Ames	TS	1		SPREFID	Sponsor's Study Reference ID		NOT APPLICABLE
9	8325064	Ames	TS	1	1	TSTFNAM	Test Facility Name	Example Tox Lab Name
10	8325064	Ames	TS	1	1	TSTFLOC	Test Facility Location	10 Somewhere Street, Montgomery, AL 10000
11	8325064	Ames	TS	1	1	TFCNTRY	Test Facility Country	USA
12	8325064	Ames	TS	1	1	STDIR	Study Director	Dr. R. Smith
13	8325064	Ames	TS	1		GLPFL	GLP Flag	N
14	8325064	Ames	TS	1		ASTD	Assay Standard	OECD TEST NO. 471
15	8325064	Ames	TS	1		ASTDV	Assay Standard Version	2020-06-29
16	8325064	Ames	TS	1		SSTYP	Study Type	GENOTOXICITY IN VITRO
17	8325064	Ames	TS	1		SSSTYP	Study Sub Type	BACTERIAL REVERSE MUTATION TEST
18	8325064	Ames	TS	1	2	SPECIES	Species	SALMONELLA TYPHIMURIUM TOBA-123 - Jira project doesn't exist or you don't have permission to view it.
19	8325064	Ames	TS	1	2	STRAIN	Strain/Substrain	TA98
20	8325064	Ames	TS	1	2	STRAIN	Strain/Substrain	TA100
21	8325064	Ames	TS	1	2	STRAIN	Strain/Substrain	TA1535
22	8325064	Ames	TS	1	2	STRAIN	Strain/Substrain	TA1537
23	8325064	Ames	TS	1	3	SPECIES	Species	Escherichia coli
24	8325064	Ames	TS	1	3	STRAIN	Strain/Substrain	WP2 uvrA pKM101

Dataset Debug Messages

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This example trial set dataset, tx.xpt, shows information about the test conditions for SetA and SetR in this example study. For brevity, this does not show information for SetF, SetG, or any other sets. A fully formed tx.xpt for this example study would include information about the test conditions for all sets.

Dataset Wrapper Debug Message

Row captions have been provided, but there doesn't seem to be a dataset to which the row captions would apply.

Rows 1-22:	Show trial set parameters and trial set values that comprise the test conditions for the set SetA. SetA is the data for the vehicle control (i.e., Strain of TA98, vehicle control with concentration value of 0). SetA is associated with the first row, labeled "SetA", in the report table for example study 8325064.
Rows 23-44:	Show trial set parameters and trial set values that comprise the test conditions for the set SetR. SetR is the data for the vehicle control (i.e., Strain of TA100, positive control with a concentration value of 2). SetR is associated with the eighteenth row, labeled "SetR", in the report table for example study 8325064.
Rows 13, 35:	Show a value for SPTOBID=CIG01a. SPTOBID is Sponsor-Defined Tobacco Product ID that is used to uniquely identify the tobacco product. The value of SPTOBID, in this case CIG01a, matches the value for SPTOBID in all the TOPARMCD-TOVAL pairs in the Tobacco Product Identifiers and Descriptors (TO) dataset example in Section 3.1.2 Product Design Parameters and Conformance Testing Example 1. The TOPARMCD-TOVAL pairs identify this unique product, CIG01a. The TO domain is described in the Section 2.8.8.1 Tobacco Product Identifiers and Descriptors (TO).
Rows 21, 43:	Show a value for SPDEVID. SPDEVID is the Sponsor defined device identifier that is used to uniquely identify the device in the Unique Device Identification (DI) dataset. The value of SPDEVID, in this case PUFFMASTER3K, matches the value of SPDEVID in all the DIPARMCD-DIVAL pairs that identify this unique device in the DI dataset. This is shown in the di.xpt in SDTM Example.Product HPHC ENDS Testing. The DI domain is described in the Section 2.8.9.7 Device Identifiers (DI).
Rows 22, 44:	Show a value for DUREFID. DUREFID serves as a link to the Device-In Use Properties (DU) domain, where a matching value of DUREFID indicates parameters of the smoking regimen performed by the smoking machine, as in the du.xpt in SDTM Example.Product HPHC ENDS Testing. The DU domain is described in the Section 2.8.9.8 Device-In-Use (DU).

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Aug 23: LAK add F & G to TX and add G data to GT.

Row	STUDYID	GNTXAID	DOMAIN	SETCD	SET	TXSEQ	TXPARMCD	TXPARM	TXVAL
1	8325064	Ames	TX	SetA	A-TA98-C0	1	SPECIES	Species	SALMONELLA TYPHIMURIUM
2	8325064	Ames	TX	SetA	A-TA98-C0	2	STRAIN	Strain/Substrain	TA98
3	8325064	Ames	TX	SetA	A-TA98-C0	3	METACT	Metabolic Activation	NOT APPLICABLE
4	8325064	Ames	TX	SetA	A-TA98-C0	4	METACTFL	Y/N presence of metabolic activation	N
5	8325064	Ames	TX	SetA	A-TA98-C0	5	TRTDMIN	Treatment Duration Minimum	71.5
6	8325064	Ames	TX	SetA	A-TA98-C0	6	TRTDTRG	Treatment Duration Target	72
7	8325064	Ames	TX	SetA	A-TA98-C0	7	TRTDMAX	Treatment Duration Maximum	72.5
8	8325064	Ames	TX	SetA	A-TA98-C0	8	TRTDU	Treatment Duration Unit	HOURS
9	8325064	Ames	TX	SetA	A-TA98-C0	9	INCBTMP	Incubation Temperature	37
10	8325064	Ames	TX	SetA	A-TA98-C0	10	INCBTMPU	Incubation Temperature Unit	C
11	8325064	Ames	TX	SetA	A-TA98-C0	11	HUMID	Atmospheric Relative Humidity Percent	50
12	8325064	Ames	TX	SetA	A-TA98-C0	12	ATMCO2	Atmospheric CO2 Percent	5
13	8325064	Ames	TX	SetA	A-TA98-C0	13	SPTOBID	Sponsor defined tobacco identifier	CIG01a
14	8325064	Ames	TX	SetA	A-TA98-C0	14	EXPTYP	Exposure Type	Submerged
15	8325064	Ames	TX	SetA	A-TA98-C0	15	SAMTYP	Sample Type	Total Particulate Matter in PBS
16	8325064	Ames	TX	SetA	A-TA98-C0	16	INTRVN	Name of the intervention article	TA1
17	8325064	Ames	TX	SetA	A-TA98-C0	17	ITVTYPE	Type of intervention article	Vehicle Control
18	8325064	Ames	TX	SetA	A-TA98-C0	18	ITVCONC	Concentration of intervention article	0
19	8325064	Ames	TX	SetA	A-TA98-C0	19	ITVCONCU	Concentration Unit	ug/plate
20	8325064	Ames	TX	SetA	A-TA98-C0	20	TRTV	Treatment Vehicle	DMSO
21	8325064	Ames	TX	SetA	A-TA98-C0	21	SPDEVID	Sponsor defined device identifier	PUFFMASTER3K
22	8325064	Ames	TX	SetA	A-TA98-C0	22	DUREFID	Smoke Regimen	NON-INTENSE REGIMEN
23	8325064	Ames	TX	SetF	F-TA98-C500	23	SPECIES	Species	SALMONELLA TYPHIMURIUM
24	8325064	Ames	TX	SetF	F-TA98-C500	24	STRAIN	Strain/Substrain	TA98
25	8325064	Ames	TX	SetF	F-TA98-C500	25	METACT	Metabolic Activation	NOT APPLICABLE
26	8325064	Ames	TX	SetF	F-TA98-C500	26	METACTFL	Y/N presence of metabolic activation	N
27	8325064	Ames	TX	SetF	F-TA98-C500	27	TRTDMIN	Treatment Duration Minimum	71.5
28	8325064	Ames	TX	SetF	F-TA98-C500	28	TRTDTRG	Treatment Duration Target	72
29	8325064	Ames	TX	SetF	F-TA98-C500	29	TRTDMAX	Treatment Duration Maximum	72.5
30	8325064	Ames	TX	SetF	F-TA98-C500	30	TRTDU	Treatment Duration Unit	HOURS
31	8325064	Ames	TX	SetF	F-TA98-C500	31	INCBTMP	Incubation Temperature	37
32	8325064	Ames	TX	SetF	F-TA98-C500	32	INCBTMPU	Incubation Temperature Unit	C
33	8325064	Ames	TX	SetF	F-TA98-C500	33	HUMID	Atmospheric Relative Humidity Percent	50
34	8325064	Ames	TX	SetF	F-TA98-C500	34	ATMCO2	Atmospheric CO2 Percent	5
35	8325064	Ames	TX	SetF	F-TA98-C500	35	SPTOBID	Sponsor defined tobacco identifier	CIG01a
36	8325064	Ames	TX	SetF	F-TA98-C500	36	EXPTYP	Exposure Type	Submerged
37	8325064	Ames	TX	SetF	F-TA98-C500	37	SAMTYP	Sample Type	Total Particulate Matter in PBS
38	8325064	Ames	TX	SetF	F-TA98-C500	38	INTRVN	Name of the intervention article	TA1
39	8325064	Ames	TX	SetF	F-TA98-C500	39	ITVTYPE	Type of intervention article	Vehicle Control
40	8325064	Ames	TX	SetF	F-TA98-C500	40	ITVCONC	Concentration of intervention article	500
41	8325064	Ames	TX	SetF	F-TA98-C500	41	ITVCONCU	Concentration Unit	ug/plate
42	8325064	Ames	TX	SetF	F-TA98-C500	42	TRTV	Treatment Vehicle	DMSO
43	8325064	Ames	TX	SetF	F-TA98-C500	43	SPDEVID	Sponsor defined device identifier	PUFFMASTER3K
44	8325064	Ames	TX	SetF	F-TA98-C500	44	DUREFID	Smoke Regimen	NON-INTENSE REGIMEN
45	8325064	Ames	TX	SetG	G-TA98-C1600	45	SPECIES	Species	SALMONELLA TYPHIMURIUM
46	8325064	Ames	TX	SetG	G-TA98-C1600	46	STRAIN	Strain/Substrain	TA98
47	8325064	Ames	TX	SetG	G-TA98-C1600	47	METACT	Metabolic Activation	NOT APPLICABLE
48	8325064	Ames	TX	SetG	G-TA98-C1600	48	METACTFL	Y/N presence of metabolic activation	N
49	8325064	Ames	TX	SetG	G-TA98-C1600	49	TRTDMIN	Treatment Duration Minimum	71.5
50	8325064	Ames	TX	SetG	G-TA98-C1600	50	TRTDTRG	Treatment Duration Target	72
51	8325064	Ames	TX	SetG	G-TA98-C1600	51	TRTDMAX	Treatment Duration Maximum	72.5
52	8325064	Ames	TX	SetG	G-TA98-C1600	52	TRTDU	Treatment Duration Unit	HOURS
53	8325064	Ames	TX	SetG	G-TA98-C1600	53	INCBTMP	Incubation Temperature	37
54	8325064	Ames	TX	SetG	G-TA98-C1600	54	INCBTMPU	Incubation Temperature Unit	C
55	8325064	Ames	TX	SetG	G-TA98-C1600		HUMID	Atmospheric Relative Humidity Percent	50
56	8325064	Ames	TX	SetG	G-TA98-C1600		ATMCO2	Atmospheric CO2 Percent	5
57	8325064	Ames	TX	SetG	G-TA98-C1600		SPTOBID	Sponsor defined tobacco identifier	CIG01a
58	8325064	Ames	TX	SetG	G-TA98-C1600		EXPTYP	Exposure Type	Submerged
59	8325064	Ames	TX	SetG	G-TA98-C1600		SAMTYP	Sample Type	Total Particulate Matter in PBS
60	8325064	Ames	TX	SetG	G-TA98-C1600		INTRVN	Name of the intervention article	TA1
61	8325064	Ames	TX	SetG	G-TA98-C1600		ITVTYPE	Type of intervention article	Vehicle Control
62	8325064	Ames	TX	SetG	G-TA98-C1600		ITVCONC	Concentration of intervention article	1600
63	8325064	Ames	TX	SetG	G-TA98-C1600		ITVCONCU	Concentration Unit	ug/plate
64	8325064	Ames	TX	SetG	G-TA98-C1600		TRTV	Treatment Vehicle	DMSO
65	8325064	Ames	TX	SetG	G-TA98-C1600		SPDEVID	Sponsor defined device identifier	PUFFMASTER3K
66	8325064	Ames	TX	SetG	G-TA98-C1600		DUREFID	Smoke Regimen	NON-INTENSE REGIMEN
67	8325064	Ames	TX	SetR	R-TA100-C2		SPECIES	Species	Salmonella typhimurium
68	8325064	Ames	TX	SetR	R-TA100-C2		STRAIN	Strain/Substrain	TA100
69	8325064	Ames	TX	SetR	R-TA100-C2		METACT	Metabolic Activation	NOT APPLICABLE
70	8325064	Ames	TX	SetR	R-TA100-C2		METACTFL	Y/N presence of metabolic activation	N
71	8325064	Ames	TX	SetR	R-TA100-C2		TRTDMIN	Treatment Duration Minimum	71.5
72	8325064	Ames	TX	SetR	R-TA100-C2		TRTDTRG	Treatment Duration Target	72
73	8325064	Ames	TX	SetR	R-TA100-C2		TRTDMAX	Treatment Duration Maximum	72.5
74	8325064	Ames	TX	SetR	R-TA100-C2		TRTDU	Treatment Duration Unit	HOURS
75	8325064	Ames	TX	SetR	R-TA100-C2		INCBTMP	Incubation Temperature	37
76	8325064	Ames	TX	SetR	R-TA100-C2		INCBTMPU	Incubation Temperature Unit	C
77	8325064	Ames	TX	SetR	R-TA100-C2		HUMID	Atmospheric Relative Humidity Percent	50
78	8325064	Ames	TX	SetR	R-TA100-C2		ATMCO2	Atmospheric CO2 Percent	5
79	8325064	Ames	TX	SetR	R-TA100-C2		SPTOBID	Sponsor defined tobacco identifier	CIG01a
80	8325064	Ames	TX	SetR	R-TA100-C2		EXPTYP	Exposure Type	Submerged
81	8325064	Ames	TX	SetR	R-TA100-C2		SAMTYP	Sample Type	Total Particulate Matter in PBS
82	8325064	Ames	TX	SetR	R-TA100-C2		INTRVN	name of the intervention article	TA1
83	8325064	Ames	TX	SetR	R-TA100-C2		ITVTYPE	type of intervention article	Positive Control
84	8325064	Ames	TX	SetR	R-TA100-C2		ITVCONC	Concentration of intervention article	2
85	8325064	Ames	TX	SetR	R-TA100-C2		ITVCONCU	Concentration Unit	ug/plate
86	8325064	Ames	TX	SetR	R-TA100-C2		TRTV	Treatment Vehicle	DMSO
87	8325064	Ames	TX	SetR	R-TA100-C2		SPDEVID	Sponsor defined device identifier	PUFFMASTER3K
88	8325064	Ames	TX	SetR	R-TA100-C2		DUREFID	Smoke Regimen	NON-INTENSE REGIMEN

REFID values are defined by the applicant to uniquely identify the observational unit within an experimental unit. In the simplified picture of an assay (below), the test tube that contains the possible mutagen is an example of an entity that would have a REFID indicating information at the level of trial set. The petri plate created from that test tube and used to count revertant numbers is an example of an entity that would be at an observational level.

This picture does not depict the more complicated procedures, trial sets, or observational units for our example study, 8325064. For study 8325064, the applicant chose to define the REFIDs at the trial set level with a single character (e.g., A, F, G, R) and chose to define REFIDs at the observational level based on the location of each tube in one of multiple exposure/incubation plates (e.g, 6_1 for plate 6, position 1).

Dataset Wrapper Debug Message

Row captions have been provided, but there doesn't seem to be a dataset to which the row captions would apply.

Row 1:	Shows the value of REFID=A, This REFID refers to the trial set with a SETCD of 'SetA', as defined in the trial set dataset, tx.xpt. LEVEL=1 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 2-6:	Show the values of five observational units 0_1 through 0_5 that are within the parent experimental unit, REFID=A
Row 7:	Shows the value of REFID=F, This REFID refers to the trial set with a SETCD of 'SetF', as defined in the trial set dataset, tx.xpt. LEVEL=1 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 8-10:	Show the values of three observational units 6_1 through 6_3 that are within the parent experimental unit, REFID=F
Row 11:	Shows the value of REFID=G, This REFID refers to the trial set with a SETCD of 'SetG', as defined in the trial set dataset, tx.xpt. LEVEL=1 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 12-14:	Show the values of three observational units 7_1 through 7_3 that are within the parent experimental unit, REFID=F
Row 15:	Shows the value of REFID=R. This REFID refers to the trial set with a SETCD of 'SetG', as defined in the trial set dataset, tx.xpt. LEVEL=1 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 16-18:	Show the values of three observational units 18_1 through 18_3 that are within the parent experimental unit, REFID=F

Unable to render dataset table

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1.For each Unique GNTXAID (For each unique value in Parent, there must be one and only one record in REFID column with a value matching that Parent value.)

"For each PARENT value within the same GNTXAID value there must be one and only one record with REFID = the value in PARENT"

2. The value of level for a record with Parent populated, that level must be one greater than it’s parent record.

Row	STUDYID	GNTXAID	SETCD	REFID	PARENT	LEVEL	LVLDESC
1	8325064	Ames	SetA	A		1	TRIAL SET
2	8325064	Ames	SetA	0_1	A	2	OBSERVATIONAL UNIT
3	8325064	Ames	SetA	0_2	A	2	OBSERVATIONAL UNIT
4	8325064	Ames	SetA	0_3	A	2	OBSERVATIONAL UNIT
5	8325064	Ames	SetA	0_4	A	2	OBSERVATIONAL UNIT
6	8325064	Ames	SetA	0_5	A	2	OBSERVATIONAL UNIT
7	8325064	Ames	SetF	F		1	TRIAL SET
8	8325064	Ames	SetF	6_1	F	2	OBSERVATIONAL UNIT
9	8325064	Ames	SetF	6_2	F	2	OBSERVATIONAL UNIT
10	8325064	Ames	SetF	6_3	F	2	OBSERVATIONAL UNIT
11	8325064	Ames	SetG	G		1	TRIAL SET
12	8325064	Ames	SetG	7_1	G	2	OBSERVATIONAL UNIT
13	8325064	Ames	SetG	7_2	G	2	OBSERVATIONAL UNIT
14	8325064	Ames	SetG	7_3	G	2	OBSERVATIONAL UNIT
15	8325064	Ames	SetG	R		1	TRIAL SET
16	8325064	Ames	SetR	18_1	R	2	OBSERVATIONAL UNIT
17	8325064	Ames	SetR	18_2	R	2	OBSERVATIONAL UNIT
18	8325064	Ames	SetR	18_3	R	2	OBSERVATIONAL UNIT

Rows 1-5:	Show the number of revertant colonies per plate collected for each of five observational units GTREFID=0_1 through 0_5 (see description in the relref dataset).
Rows 6, 7:	Show summary values collected (MEAN, STANDARD DEVIATION) for GTREFID=A that apply to the entire trial set, SetA, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID as shown in the relref dataset.
Rows 8-13:	Revertent colonies were counted for each of three plates/observational units (GTREFID=6_1 through 6_3) and each value is associated with a record to show a postfix code of "V" = "Very thin background bacterial lawn"
Rows 14-16:	Show summary values collected (MEAN, STANDARD DEVIATION, and FOLD INCREASE) for GTREFID=F that apply to the entire trial set, SetF, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID in the relref dataset.
Rows 17-19:	Show three plates/observational units (GTREFID=5_1 through 5_3) where no revertant colonies were counted due to too much cytotoxicity and a postfix code of "T".
Rows 20-22:	Show the number of revertant colonies per plate collected for each of three observational units (GTREFID=18_1 through 18_3).
Rows 23-25:	Show summary values collected (MEAN, STANDARD DEVIATION, and FOLD INCREASE) for GTREFID=R that apply to the entire trial set, SetR, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID in the relref dataset.

gt.xpt

Row	STUDYID	GNTXAID	DOMAIN	GTSEQ	GTREFID	GTTESTCD	GTTEST	GTORRES	GTORRESU	GTCOLSRT	GTSTRESC	GTSTRESN	GTSTRESU	GTSTAT	GTREASND	GTMETHOD	GTDTC
1	8325064	Ames	GT	1	0_1	RPP	Revertant Colony Numbers Per Plate	26	NOT APPLICABLE		26	26	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
2	8325064	Ames	GT	2	0_2	RPP	Revertant Colony Numbers Per Plate	35	NOT APPLICABLE		35	35	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
3	8325064	Ames	GT	3	0_3	RPP	Revertant Colony Numbers Per Plate	39	NOT APPLICABLE		39	39	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
4	8325064	Ames	GT	4	0_4	RPP	Revertant Colony Numbers Per Plate	35	NOT APPLICABLE		35	35	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
5	8325064	Ames	GT	5	0_5	RPP	Revertant Colony Numbers Per Plate	30	NOT APPLICABLE		30	30	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
6	8325064	Ames	GT	6	A	RPP	Revertant Colony Numbers Per Plate	33.0	NOT APPLICABLE	MEAN	33.0	33.0	NOT APPLICABLE				2015-08-03
7	8325064	Ames	GT	7	A	RPP	Revertant Colony Numbers Per Plate	5.0	NOT APPLICABLE	STANDARD DEVIATION	5.0	5.0	NOT APPLICABLE				2015-08-03
8	8325064	Ames	GT	1	6_1	RPP	Revertant Colony Numbers Per Plate	8	NOT APPLICABLE		8	8	NOT APPLICABLE			MANUALLY COUNTED	2015-08-03
9	8325064	Ames	GT	2	6_1	CYTOTOX	Cytotoxicity	Very thin background bacterial lawn			V						2015-08-03
10	8325064	Ames	GT	3	6_2	RPP	Revertant Colony Numbers Per Plate	10	NOT APPLICABLE		10	10	NOT APPLICABLE			MANUALLY COUNTED	2015-08-03
11	8325064	Ames	GT	4	6_2	CYTOTOX	Cytotoxicity	Very thin background bacterial lawn			V						2015-08-03
12	8325064	Ames	GT	5	6_3	RPP	Revertant Colony Numbers Per Plate	12	NOT APPLICABLE		12	12	NOT APPLICABLE			MANUALLY COUNTED	2015-08-03
13	8325064	Ames	GT	6	6_3	CYTOTOX	Cytotoxicity	Very thin background bacterial lawn			V						2015-08-03
14	8325064	Ames	GT	7	F	RPPMEAN	Mean Rev Colony Num Per Plate	10.0	NOT APPLICABLE	MEAN	10.0	10.0	NOT APPLICABLE				2015-08-03
15	8325064	Ames	GT	8	F	RPPSTDDV	Std Dev Rev Colony Num Per Plate	2.0	NOT APPLICABLE	STANDARD DEVIATION	2.0	2.0	NOT APPLICABLE				2015-08-03
16	8325064	Ames	GT	9	F	RPPFLDIC	Fold Increase Rev Colony Num Per Plate	0.3	NOT APPLICABLE	FOLD INCREASE	0.3	0.3	NOT APPLICABLE				2015-08-03
17	8325064	Ames	GT	1	5_1	CYTOTOX	Cytotoxicity	Toxic No Revertant Colonies			T			NOT DONE	TOO MUCH CYTOTOXICITY		2015-08-03
18	8325064	Ames	GT	2	5_2	CYTOTOX	Cytotoxicity	Toxic No Revertant Colonies			T			NOT DONE	TOO MUCH CYTOTOXICITY		2015-08-03
19	8325064	Ames	GT	3	5_3	CYTOTOX	Cytotoxicity	Toxic No Revertant Colonies			T			NOT DONE	TOO MUCH CYTOTOXICITY		2015-08-03
20	8325064	Ames	GT	1	18_1	RPP	Revertant Colony Numbers Per Plate	812	NOT APPLICABLE		812	812	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
21	8325064	Ames	GT	2	18_2	RPP	Revertant Colony Numbers Per Plate	912	NOT APPLICABLE		912	912	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
22	8325064	Ames	GT	3	18_3	RPP	Revertant Colony Numbers Per Plate	839	NOT APPLICABLE		839	839	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
23	8325064	Ames	GT	4	R	RPPMEAN	Mean Rev Colony Num Per Plate	854.3	NOT APPLICABLE	MEAN	854.3	854.3	NOT APPLICABLE				2015-08-03
24	8325064	Ames	GT	5	R	RPPSTDDV	Std Dev Rev Colony Num Per Plate	51.7	NOT APPLICABLE	STANDARD DEVIATION	51.7	51.7	NOT APPLICABLE				2015-08-03
25	8325064	Ames	GT	6	R	RPPFLDIC	Fold Increase Rev Colony Num Per Plate	6.4	NOT APPLICABLE	FOLD INCREASE	6.4	6.4	NOT APPLICABLE				2015-08-03

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