Tags: FISH, EGFR, Cytogenetic, cytogenetics
This example is currently in draft. |
This example shows findings from a cytogenetic assessment of EGFR gene copy number amplification with the purpose of determining the diagnostic status of patients with non-small cell lung cancer. In this example, the findings are generated using fluorescence in situ hybridization (FISH) methodology and semi-quantitative microscopic assessment by a pathologist. Microscopic assessments are then summarized to a diagnostic status using a specific algorithm represented in GFANMTH.
| Rows 1, 5: | Show the total number of centromere probe signals in the nuclei of the counted cells associated with the location represented in variable GFGENLOC for chromosome 7. | | Rows 2, 6: | Show the total number of EGFR probe signals in the nuclei of the counted cells. | | Rows 3, 7: | Show the calculated ratio of the EGFR probe signals to centromere probe signals. | | Rows 4, 8: | Show the EGFR amplification status of the sample according to the 2009 Colorado Scoring Method for EGFR Gene Amplification Algorithm. |
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| Row | STUDYID | DOMAIN | USUBJID | GFSEQ | GFREFID | GFTESTCD | GFTEST | GFTSTDTL | GFORRES | GFORRESU | GFSTRESC | GFSTRESN | GFSTRESU | GFCHROM | GFSYM | GFSYMTYP | GFGENLOC | GFNAM | GFSPEC | GFMETHOD | GFANMETH | VISITNUM | VISIT | VISITDY | GFDTC | GFDY | | 1 | ABC0023 | GF | AB23-001 | 1 | 45785-01 | CPNUMAMP | Copy Number Amplification | CENTROMERE SIGNALS | 147 | /50 Cells | 147 | 147 | /50 Cells | 7 |
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| 7p11.1-q11.1 | ACME GENOMICS LABS | DNA | FISH |
| -1 | SCREENING | -2 | 6/21/2023 | -1 | | 2 | ABC0023 | GF | AB23-001 | 2 | 45785-01 | CPNUMAMP | Copy Number Amplification | GENOME-SPECIFIC TARGET SIGNALS | 166 | /50 Cells | 166 | 166 | /50 Cells | 7 | EGFR | GENE WITH PROTEIN PRODUCT |
| ACME GENOMICS LABS | DNA | FISH |
| -1 | SCREENING | -2 | 6/21/2023 | -1 | | 3 | ABC0023 | GF | AB23-001 | 3 | 45785-01 | CPNUMAMP | Copy Number Amplification | COPY NUMBER RATIO | 1.1 |
| 1.1 | 1.1 |
| 7 | EGFR | GENE WITH PROTEIN PRODUCT |
| ACME GENOMICS LABS | DNA | FISH |
| -1 | SCREENING | -2 | 6/21/2023 | -1 | | 4 | ABC0023 | GF | AB23-001 | 4 | 45785-01 | CPNUMAMP | Copy Number Amplification | DETECTION | NEGATIVE |
| NEGATIVE |
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| 7 | EGFR | GENE WITH PROTEIN PRODUCT |
| ACME GENOMICS LABS | DNA | FISH | COLORADO SCORING METHOD FOR EGFR GENE AMPLIFICATION 2009 ALGORITHM | -1 | SCREENING | -2 | 6/21/2023 | -1 |
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| Copy Number Amplification | CENTROMERE SIGNALS | 165 |
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| Copy Number Amplification | GENOME-SPECIFIC TARGET SIGNALS | 208 |
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| Copy Number Amplification | COPY NUMBER RATIO | 1.3 |
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| Copy Number Amplification | DETECTION | POSITIVE |
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