SEND Example.In vitro Bacterial Reverse Mutation Assay

This is an example showing the report table, trial design, and results from the in vitro bacterial reverse mutation test of an example study, Study #8325064. This bacterial reverse mutation test uses four different amino acid-requiring strains of Salmonella typhimurium (S. typhimurium) and one strain of Escherichia coli (E. coli) to detect point mutations, which involve substitution, addition or deletion of one or a few DNA base pairs. TOBA-129 - Getting issue details... STATUS

In this example, the Trial Summary dataset, TOBA-368 - Getting issue details... STATUS ts.xpt, includes many informational fields that may provide context at the study level, for Study 8325064. Also, TSGRPID has been used to link records (name, location, country) related to the test facility (TSGRPID = 1) and records related to the Test Site (TSGRPID = 2). The Study Director is associated with the Test Facility and the Principal Investigator is associated with the Test Site. Finally, the Primary Treatment CAS Registry Number is not known and this is recorded as an empty TSVAL and UNKNOWN in the corresponding TSVALNF.

This table shows the assay results for Study 8325064, Test Article TA1, and three strains of Salmonella (TA98, TA100, and TA1535) at varying concentrations. For ease of reference, each row has been labeled in the right hand margin with the applicant-defined trial set label (e.g., SetA). For brevity, the remaining tables (e.g., additional strains, samples prepared without metabolic activation, etc.) are not included.

Rows 1-2:	Show two records for TSPARMCD = "GLPTYP", using TSSEQ to indicate multiple records, TOBA-366 - Jira project doesn't exist or you don't have permission to view it. since both GLP Types apply for this example study.
Row 8:	Shows that the applicant's study reference ID is not applicable.
Rows 9-12:	Show that TSGRPID has been used to link records (name, location, country) related to the test facility (TSGRPID = 1). The Study Director is associated with the Test Facility.
Rows 14-16:	Show that TSGRPID (TSGRPID=4) has been used to link the information on the testing guideline followed on this study (TSTGDNAM, TSTGDORG, TSTGDVER). TOBA-56 - Jira project doesn't exist or you don't have permission to view it.
Rows 18-26: TOBA-367 - Jira project doesn't exist or you don't have permission to view it.	Show that this example study includes two different species of bacteria and a total of five strains.
Rows 18-23:	Show that TSGRPID (TSGRPID = 2) has been used to link the information for one species (Salmonella) with four different strains that are tested in this example study.
Rows 24-26:	Show that TSGRPID (TSGRPID = 3) has been used to link the information for one species (Escherichia coli) with the strain that is tested in this example study.

Row Captions Debug Message

Please move all content other than the row caption labels out of the first column of the table.

ts.xpt

xx.xpt

Row	STUDYID	GNTXAID	DOMAIN	TSSEQ	TSGRPID	TSPARMCD	TSPARM	TSVAL	TSVALNF
1	8325064	Ames	TS	1		GLPTYP	Good Laboratory Practice Type	FDA
2	8325064	Ames	TS	2		GLPTYP	Good Laboratory Practice Type	OECD
3	8325064	Ames	TS	1		STSTDTC	Study Start Date	2015-07-29
4	8325064	Ames	TS	1		STITLE	Study Title	The Bacterial Reverse Mutation Test, Study 8325064-1
5	8325064	Ames	TS	1		SNDIGVER	SEND Implementation Guide Version	TOBACCO IMPLEMENTATION GUIDE VERSION 1.0
6	8325064	Ames	TS	1		SNDCTVER	SEND Controlled Terminology Version	SEND Terminology 2021-09-30
7	8325064	Ames	TS	1		SAPPLCNT	Applicant Organization	Example Applicant Inc.
8	8325064	Ames	TS	1		SPREFID	Applicant's Study Reference ID		NOT APPLICABLE
9	8325064	Ames	TS	1	1	TSTFNAM	Test Facility Name	Example Tox Lab Name
10	8325064	Ames	TS	1	1	TSTFLOC	Test Facility Location	10 Somewhere Street, Montgomery, AL 10000
11	8325064	Ames	TS	1	1	TFCNTRY	Test Facility Country	USA
12	8325064	Ames	TS	1	1	STDIR	Study Director	Dr. R. Smith
13	8325064	Ames	TS	1		GLPFL	GLP Flag	N
14	8325064	Ames	TS	1	4	TSTGDNAM	Testing Guideline Name	Test NO. 471
15	8325064	Ames	TS	1	4	TSTGDORG	Testing Guideline Organization	OECD
16	8325064	Ames	TS	1	4	TSTGDVER	Testing Guideline Version	2020-06-29
17	8325064	Ames	TS	1		SSTYP	Study Type	GENOTOXICITY IN VITRO
18	8325064	Ames	TS	1		SSSTYP	Study Sub Type	BACTERIAL REVERSE MUTATION TEST
19	8325064	Ames	TS	1	2	SPECIES	Species	SALMONELLA TYPHIMURIUM TOBA-123 - Jira project doesn't exist or you don't have permission to view it.
20	8325064	Ames	TS	1	2	STRAIN	Strain/Substrain	TA98
21	8325064	Ames	TS	1	2	STRAIN	Strain/Substrain	TA100
22	8325064	Ames	TS	1	2	STRAIN	Strain/Substrain	TA1535
23	8325064	Ames	TS	1	2	STRAIN	Strain/Substrain	TA1537
24	8325064	Ames	TS	1	2	CELLLN	Cell Line
25	8325064	Ames	TS	1	3	SPECIES	Species	Escherichia coli
26	8325064	Ames	TS	1	3	STRAIN	Strain/Substrain	WP2 uvrA pKM101
27	8325064	Ames	TS	1	3	CELLLN	Cell Line

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This example trial set dataset, tx.xpt, shows information about the test conditions for SetA and SetR in this example study. For brevity, this does not show information for SetF, SetG, or any other sets. A fully formed tx.xpt for this example study would include information about the test conditions for all sets.

Rows 1-14:	Show trial set, SetSTM, with trial set parameters and trial set values that comprise the test conditions for all data for the species Salmonella Typhimurium.
Rows 15, 24, 33, 42:	Show that SetA, SetF, SetG, and SetR each have a parent set code of SetSTM. As a child set of SetSTM, each of these sets inherit the test conditions of SetSTM.
Rows 16-23:	Show trial set parameters and trial set values that comprise the test conditions for the set SetA. SetA is the data for the vehicle control (i.e., Strain of TA98, vehicle control with concentration value of 0). SetA is associated with the first row, labeled "SetA", in the report table for example study 8325064.
Rows 25-32:	Show trial set parameters and trial set values that comprise the test conditions for the set SetF. SetF is the data for the strain of TA98 with a concentration value of 500 µg/plate. SetF is associated with the sixth row, labeled "SetF", in the report table for example study 8325064.
Rows 33-41:	Show trial set parameters and trial set values that comprise the test conditions for the set SetG. SetG is the data for the strain of TA98 with a concentration value of 1600 µg/plate. SetG is associated with the seventh row, labeled "SetG", in the report table for example study 8325064.
Rows 42-50:	Show trial set parameters and trial set values that comprise the test conditions for the set SetR. SetR is the data for the strain of TA100, positive control with a concentration value of 2 µg/plate. SetR is associated with the eighteenth row, labeled "SetR", in the report table for example study 8325064.
Row 10:	Shows a value for SPTOBID=CIG01a. SPTOBID is Applicant-Defined Tobacco Product ID that is used to uniquely identify the tobacco product. The value of SPTOBID, in this case CIG01a, matches the value for SPTOBID in all the TOPARMCD-TOVAL pairs in the Tobacco Product Identifiers and Descriptors (TO) dataset example in Section 3.1.2 Product Design Parameters and Conformance Testing Example 1. The TOPARMCD-TOVAL pairs identify this unique product, CIG01a. The TO domain is described in the Section 2.8.8.1 Tobacco Product Identifiers and Descriptors (TO).
Row 13:	Shows a value for SPDEVID. SPDEVID is the applicant-defined device identifier that is used to uniquely identify the device in the Unique Device Identification (DI) dataset. The value of SPDEVID, in this case PUFFMASTER3K, matches the value of SPDEVID in all the DIPARMCD-DIVAL pairs that identify this unique device in the DI dataset. This is shown in the di.xpt in SDTM Example.Product HPHC ENDS Testing. The DI domain is described in the Section 2.8.9.7 Device Identifiers (DI).
Row 14:	Shows a value for DUREFID. DUREFID serves as a link to the Device-In Use Properties (DU) domain, where a matching value of DUREFID indicates parameters of the smoking regimen performed by the smoking machine, as in the du.xpt in SDTM Example.Product HPHC ENDS Testing. The DU domain is described in the Section 2.8.9.8 Device-In-Use (DU).

tx.xpt

Row	STUDYID	GNTXAID	DOMAIN	SETCD	SET	TXSEQ	TXPARMCD	TXPARM	TXVAL
1	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	1	SPECIES	Species	SALMONELLA TYPHIMURIUM
2	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	2	TRTDMIN	In vitro Treatment Duration Minimum	71.5
3	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	3	TRTDTRG	In vitro Treatment Duration Target	72
4	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	4	TRTDMAX	In vitro Treatment Duration Maximum	72.5
5	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	5	TRTDU	In vitro Treatment Duration Unit	HOURS
6	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	6	INCBTMP	Incubation Temperature	37
7	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	7	INCBTMPU	Incubation Temperature Unit	C
8	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	8	HUMID	Atmospheric Relative Humidity Percent	50
9	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	9	ATMCO2	Atmospheric CO2 Percent	5
10	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	10	SPTOBID	Applicant-defined tobacco identifier	CIG01a
11	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	11	EXPTYP	Exposure Type	Submerged
12	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	12	SAMTYP	Sample Type	Total Particulate Matter in PBS
13	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	13	SPDEVID	Applicant-defined device identifier	PUFFMASTER3K
14	8325064	Ames	TX	SetSTM	SALMONELLA TYPHIMURIUM	14	DUREFID	Smoke Regimen	NON-INTENSE REGIMEN
15	8325064	Ames	TX	SetA	A-TA98-C0	15	PSETCD	Parent Set Code	SetSTM
16	8325064	Ames	TX	SetA	A-TA98-C0	15	STRAIN	Strain/Substrain	TA98
17	8325064	Ames	TX	SetA	A-TA98-C0	16	METACT	Metabolic Activation	NOT APPLICABLE
18	8325064	Ames	TX	SetA	A-TA98-C0	17	METACTFL	Y/N presence of metabolic activation	N
19	8325064	Ames	TX	SetA	A-TA98-C0	18	INTRVN	Name of the intervention article	TA1
20	8325064	Ames	TX	SetA	A-TA98-C0	19	ITVTYPE	Type of intervention article	Vehicle Control
21	8325064	Ames	TX	SetA	A-TA98-C0	20	ITVCONC	Concentration of intervention article	0
22	8325064	Ames	TX	SetA	A-TA98-C0	21	ITVCONCU	Concentration Unit	ug/plate
23	8325064	Ames	TX	SetA	A-TA98-C0	22	TRTV	Treatment Vehicle	DMSO
24	8325064	Ames	TX	SetF	F-TA98-C500		PSETCD	Parent Set Code	SetSTM
25	8325064	Ames	TX	SetF	F-TA98-C500	23	STRAIN	Strain/Substrain	TA98
26	8325064	Ames	TX	SetF	F-TA98-C500	24	METACT	Metabolic Activation	NOT APPLICABLE
27	8325064	Ames	TX	SetF	F-TA98-C500	25	METACTFL	Y/N presence of metabolic activation	N
28	8325064	Ames	TX	SetF	F-TA98-C500	26	INTRVN	Name of the intervention article	TA1
29	8325064	Ames	TX	SetF	F-TA98-C500	27	ITVTYPE	Type of intervention article	Product
30	8325064	Ames	TX	SetF	F-TA98-C500	28	ITVCONC	Concentration of intervention article	500
31	8325064	Ames	TX	SetF	F-TA98-C500	29	ITVCONCU	Concentration Unit	ug/plate
32	8325064	Ames	TX	SetF	F-TA98-C500	30	TRTV	Treatment Vehicle	DMSO
33	8325064	Ames	TX	SetG	G-TA98-C1600	31	PSETCD	Parent Set Code	SetSTM
34	8325064	Ames	TX	SetG	G-TA98-C1600	31	STRAIN	Strain/Substrain	TA98
35	8325064	Ames	TX	SetG	G-TA98-C1600	32	METACT	Metabolic Activation	NOT APPLICABLE
36	8325064	Ames	TX	SetG	G-TA98-C1600	33	METACTFL	Y/N presence of metabolic activation	N
37	8325064	Ames	TX	SetG	G-TA98-C1600	34	INTRVN	Name of the intervention article	TA1
38	8325064	Ames	TX	SetG	G-TA98-C1600	35	ITVTYPE	Type of intervention article	Product
39	8325064	Ames	TX	SetG	G-TA98-C1600	36	ITVCONC	Concentration of intervention article	1600
40	8325064	Ames	TX	SetG	G-TA98-C1600	37	ITVCONCU	Concentration Unit	ug/plate
41	8325064	Ames	TX	SetG	G-TA98-C1600	38	TRTV	Treatment Vehicle	DMSO
42	8325064	Ames	TX	SetR	R-TA100-C2	39	PSETCD	Parent Set Code	SetSTM
43	8325064	Ames	TX	SetR	R-TA100-C2	39	STRAIN	Strain/Substrain	TA100
44	8325064	Ames	TX	SetR	R-TA100-C2	40	METACT	Metabolic Activation	NOT APPLICABLE
45	8325064	Ames	TX	SetR	R-TA100-C2	41	METACTFL	Y/N presence of metabolic activation	N
46	8325064	Ames	TX	SetR	R-TA100-C2	42	INTRVN	name of the intervention article	NaN3
47	8325064	Ames	TX	SetR	R-TA100-C2	43	ITVTYPE	type of intervention article	Positive Control
48	8325064	Ames	TX	SetR	R-TA100-C2	44	ITVCONC	Concentration of intervention article	2
49	8325064	Ames	TX	SetR	R-TA100-C2	45	ITVCONCU	Concentration Unit	ug/plate
50	8325064	Ames	TX	SetR	R-TA100-C2	46	TRTV	Treatment Vehicle	DMSO

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Dataset Wrapper Debug Message

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REFID values are defined by the applicant to uniquely identify the observational unit within an experimental unit. In the simplified picture of an assay (below), the test tube that contains the possible mutagen is an example of an entity that would have a REFID indicating information at the level of trial set. The petri plate created from that test tube and used to count revertant numbers is an example of an entity that would be at an observational level.

This picture does not depict the more complicated procedures, trial sets, or observational units for our example study, 8325064. For study 8325064, the applicant chose to define the REFIDs at the trial set level with a single character (e.g., A, F, G, R) and chose to define REFIDs at the observational level based on the location of each tube in one of multiple exposure/incubation plates (e.g, 6_1 for plate 6, position 1).

Row 1:	Shows the value of REFID=STM, This REFID refers to the trial set with a SETCD of 'SetSTM', as defined in the trial set dataset, tx.xpt. LEVEL=1 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 1, 2, 8, 12, 16:	Show that the trial set, SetSTM, is the parent of set codes SetA, SetF, SetG, and SetR.
Row 2:	Shows the value of REFID=A, This REFID refers to the trial set with a SETCD of 'SetA', as defined in the trial set dataset, tx.xpt. LEVEL=2 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 3-7:	Show the values of five observational units 1_1 through 1_5 that are within the parent experimental unit, REFID=A
Row 8:	Shows the value of REFID=F, This REFID refers to the trial set with a SETCD of 'SetF', as defined in the trial set dataset, tx.xpt. LEVEL=2 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 9-11:	Show the values of three observational units 6_1 through 6_3 that are within the parent experimental unit, REFID=F
Row 12:	Shows the value of REFID=G, This REFID refers to the trial set with a SETCD of 'SetG', as defined in the trial set dataset, tx.xpt. LEVEL=2 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 13-15:	Show the values of three observational units 7_1 through 7_3 that are within the parent experimental unit, REFID=G
Row 16:	Shows the value of REFID=R. This REFID refers to the trial set with a SETCD of 'SetG', as defined in the trial set dataset, tx.xpt. LEVEL=2 and LVLDESC='TRIAL SET' indicates this identifier relates to the entire trial set.
Rows 17-19:	Show the values of three observational units 18_1 through 18_3 that are within the parent experimental unit, REFID=R

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relref.xpt

Row	STUDYID	GNTXAID	SETCD	REFID	PARENT	LEVEL	LVLDESC
1	8325064	Ames	SetSTM	STM		1	TRIAL SET
2	8325064	Ames	SetA	A	STM	2	EXPERIMENTAL UNIT/TRIAL SET
3	8325064	Ames	SetA	1_1	A	3	OBSERVATIONAL UNIT
4	8325064	Ames	SetA	1_2	A	3	OBSERVATIONAL UNIT
5	8325064	Ames	SetA	1_3	A	3	OBSERVATIONAL UNIT
6	8325064	Ames	SetA	1_4	A	3	OBSERVATIONAL UNIT
7	8325064	Ames	SetA	1_5	A	3	OBSERVATIONAL UNIT
8	8325064	Ames	SetF	F	STM	2	EXPERIMENTAL UNIT/TRIAL SET
9	8325064	Ames	SetF	6_1	F	3	OBSERVATIONAL UNIT
10	8325064	Ames	SetF	6_2	F	3	OBSERVATIONAL UNIT
11	8325064	Ames	SetF	6_3	F	3	OBSERVATIONAL UNIT
12	8325064	Ames	SetG	G	STM	2	EXPERIMENTAL UNIT/TRIAL SET
13	8325064	Ames	SetG	7_1	G	3	OBSERVATIONAL UNIT
14	8325064	Ames	SetG	7_2	G	3	OBSERVATIONAL UNIT
15	8325064	Ames	SetG	7_3	G	3	OBSERVATIONAL UNIT
16	8325064	Ames	SetG	R	STM	2	EXPERIMENTAL UNIT/TRIAL SET
17	8325064	Ames	SetR	18_1	R	3	OBSERVATIONAL UNIT
18	8325064	Ames	SetR	18_2	R	3	OBSERVATIONAL UNIT
19	8325064	Ames	SetR	18_3	R	3	OBSERVATIONAL UNIT

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Rows 1-5:	Show the number of revertant colonies per plate collected for each of five observational units GTREFID=1_1 through 1_5 (see description in the relref dataset).
Rows 6, 7:	Show summary values collected (MEAN, STANDARD DEVIATION) for GTREFID=A that apply to the entire trial set, SetA, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID as shown in the relref dataset.
Rows 8-13:	Revertent colonies were counted for each of three plates/observational units (GTREFID=6_1 through 6_3) and each value is associated with a record to show a postfix code of "V" = "Very thin background bacterial lawn"
Rows 14-16:	Show summary values collected (MEAN, STANDARD DEVIATION, and FOLD INCREASE) for GTREFID=F that apply to the entire trial set, SetF, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID in the relref dataset.
Rows 17-19:	Show three plates/observational units (GTREFID=5_1 through 5_3) where no revertant colonies were counted due to too much cytotoxicity and a postfix code of "T".
Rows 20-22:	Show the number of revertant colonies per plate collected for each of three observational units (GTREFID=18_1 through 18_3).
Rows 23-25:	Show summary values collected (MEAN, STANDARD DEVIATION, and FOLD INCREASE) for GTREFID=R that apply to the entire trial set, SetR, as indicated by LEVEL=1 and LVLDESC=TRIAL SET for this REFID in the relref dataset.

gt.xpt

Row	STUDYID	GNTXAID	DOMAIN	GTSEQ	GTREFID	GTTESTCD	GTTEST	GTORRES	GTORRESU	GTCOLSRT	GTSTRESC	GTSTRESN	GTSTRESU	GTSTAT	GTREASND	GTMETHOD	GTDTC
1	8325064	Ames	GT	1	1_1	RPP	Revertant Colony Numbers Per Plate	26	NOT APPLICABLE		26	26	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
2	8325064	Ames	GT	2	1_2	RPP	Revertant Colony Numbers Per Plate	35	NOT APPLICABLE		35	35	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
3	8325064	Ames	GT	3	1_3	RPP	Revertant Colony Numbers Per Plate	39	NOT APPLICABLE		39	39	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
4	8325064	Ames	GT	4	1_4	RPP	Revertant Colony Numbers Per Plate	35	NOT APPLICABLE		35	35	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
5	8325064	Ames	GT	5	1_5	RPP	Revertant Colony Numbers Per Plate	30	NOT APPLICABLE		30	30	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
6	8325064	Ames	GT	6	A	RPP	Revertant Colony Numbers Per Plate	33.0	NOT APPLICABLE	MEAN	33.0	33.0	NOT APPLICABLE				2015-08-03
7	8325064	Ames	GT	7	A	RPP	Revertant Colony Numbers Per Plate	5.0	NOT APPLICABLE	STANDARD DEVIATION	5.0	5.0	NOT APPLICABLE				2015-08-03
8	8325064	Ames	GT	1	6_1	RPP	Revertant Colony Numbers Per Plate	8	NOT APPLICABLE		8	8	NOT APPLICABLE			MANUALLY COUNTED	2015-08-03
9	8325064	Ames	GT	2	6_1	CYTOTOX	Cytotoxicity	Very thin background bacterial lawn			V						2015-08-03
10	8325064	Ames	GT	3	6_2	RPP	Revertant Colony Numbers Per Plate	10	NOT APPLICABLE		10	10	NOT APPLICABLE			MANUALLY COUNTED	2015-08-03
11	8325064	Ames	GT	4	6_2	CYTOTOX	Cytotoxicity	Very thin background bacterial lawn			V						2015-08-03
12	8325064	Ames	GT	5	6_3	RPP	Revertant Colony Numbers Per Plate	12	NOT APPLICABLE		12	12	NOT APPLICABLE			MANUALLY COUNTED	2015-08-03
13	8325064	Ames	GT	6	6_3	CYTOTOX	Cytotoxicity	Very thin background bacterial lawn			V						2015-08-03
14	8325064	Ames	GT	7	F	RPPMEAN	Mean Rev Colony Num Per Plate	10.0	NOT APPLICABLE	MEAN	10.0	10.0	NOT APPLICABLE				2015-08-03
15	8325064	Ames	GT	8	F	RPPSTDDV	Std Dev Rev Colony Num Per Plate	2.0	NOT APPLICABLE	STANDARD DEVIATION	2.0	2.0	NOT APPLICABLE				2015-08-03
16	8325064	Ames	GT	9	F	RPPFLDIC	Fold Increase Rev Colony Num Per Plate	0.3	NOT APPLICABLE	FOLD INCREASE	0.3	0.3	NOT APPLICABLE				2015-08-03
17	8325064	Ames	GT	1	7_1	CYTOTOX	Cytotoxicity	Toxic No Revertant Colonies			T			NOT DONE	TOO MUCH CYTOTOXICITY		2015-08-03
18	8325064	Ames	GT	2	7_2	CYTOTOX	Cytotoxicity	Toxic No Revertant Colonies			T			NOT DONE	TOO MUCH CYTOTOXICITY		2015-08-03
19	8325064	Ames	GT	3	7_3	CYTOTOX	Cytotoxicity	Toxic No Revertant Colonies			T			NOT DONE	TOO MUCH CYTOTOXICITY		2015-08-03
20	8325064	Ames	GT	1	18_1	RPP	Revertant Colony Numbers Per Plate	812	NOT APPLICABLE		812	812	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
21	8325064	Ames	GT	2	18_2	RPP	Revertant Colony Numbers Per Plate	912	NOT APPLICABLE		912	912	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
22	8325064	Ames	GT	3	18_3	RPP	Revertant Colony Numbers Per Plate	839	NOT APPLICABLE		839	839	NOT APPLICABLE			INSTRUMENT COUNTED	2015-08-03
23	8325064	Ames	GT	4	R	RPPMEAN	Mean Rev Colony Num Per Plate	854.3	NOT APPLICABLE	MEAN	854.3	854.3	NOT APPLICABLE				2015-08-03
24	8325064	Ames	GT	5	R	RPPSTDDV	Std Dev Rev Colony Num Per Plate	51.7	NOT APPLICABLE	STANDARD DEVIATION	51.7	51.7	NOT APPLICABLE				2015-08-03
25	8325064	Ames	GT	6	R	RPPFLDIC	Fold Increase Rev Colony Num Per Plate	6.4	NOT APPLICABLE	FOLD INCREASE	6.4	6.4	NOT APPLICABLE				2015-08-03

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SEND Example.In vitro Bacterial Reverse Mutation Assay