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title	Sample from Report Table

The following shows example data for study 123 in a report table. This report includes summary statistics (i.e., average, standard deviation, coefficient variation) for each plate and an EC50

Jira

showSummary	false
server	Issue Tracker (JIRA)
serverId	85506ce4-3cb3-3d91-85ee-f633aaaf4a45
key	TOBA-593

value for the entire assay. The grey cells in In the table below, the light grey shading for plate 1 are cells values from wells that do not contain the NHK cells. The dark grey shading represents the column and row location within the 96-well plate, and in this example the applicant chose to use these column and row locations to assign their REFID values in the RELREF dataset and GT dataset.

Neutral Red Cytotoxicity Assay Results (Relative Absorbance Data)
	Sample ID	Smoke Regime	Replicate Number	Plate Number			Relative Assay Plate Absorbance Readings
							Cigarette Smoke Condensate (µg/mL)								SLS (µg/mL)			EC50 (µg/ml)
					Well Column:	1	2	3	4	5	6	7	8	9	10	11	12
					Well Row:	0	10	50	75	100	120	140	160	200	110	200	0
	030001	Medium	1	1	A	0.046*	0.046*	0.039*	0.012*	0.030*	0.003*	0.057*	0.032*	0.003*	0.050*	0.044*	0.040*
	030001	Medium	1	1	B	0.048*	107	77.7	65.7	30.2	15.6	9.79	5.41	2.49	8.09	0.791	0.014*
	030001	Medium	1	1	C	0.005*	98.6	77.4	57.7	35.8	8.09	7.36	3.22	0.791	6.38	0.061	0.050*
	030001	Medium	1	1	D	0.049*	94.9	81.5	38.2	31.2	7.60	5.17	3.22	0.791	5.17	0.304	0.017*
	030001	Medium	1	1	E	0.047*	111	78.1	64.5	32.9	8.82	5.90	2.74	1.28	10.3	0.547	0.011*
	030001	Medium	1	1	F	0.032*	96.6	76.2	48.0	26.8	7.84	4.20	2.74	1.52	8.09	4.93	0.022*
	030001	Medium	1	1	G	0.044*	105	77.9	58.2	28.3	8.09	4.68	1.76	0.547	8.57	1.76	0.056*
	030001	Medium	1	1	H	0.056*	0.013*	0.040*	0.008*	0.013*	0.002*	0.029*	0.018*	0.006*	0.016*	0.024*	0.046*
Average							101	78.2	54.5	29.9	9.70	6.17	3.37	1.19	8.94	1.22
Std. Dev.							6	2.9	9.4	3.6	2.95	2.19	1.12	0.72	2.72	1.58
Coeff. Var.							5.8	3.6	17.2	11.9	30.4	35.5	33.1	61.0	30.5	129.9
*media without cells
	030001	Medium	1	2	A		93.4	74.0	56.0	35.1	11.8	8.70	5.10	1.98	5.34	1.02
	030001	Medium	1	2	B		102	75.2	53.3	35.1	11.8	7.74	5.58	3.66	5.34	1.02
	030001	Medium	1	2	C		107	79.0	55.0	26.5	10.1	6.06	3.66	1.74	7.02	0.780
	030001	Medium	1	2	D		104	80.2	55.3	29.8	8.94	3.90	3.66	1.50	6.30	0.780
	030001	Medium	1	2	E		101	75.7	54.1	26.7	4.86	4.38	1.26	1.02	6.30	1.02
	030001	Medium	1	2	F		110	85.7	67.7	26.0	5.34	4.62	3.42	1.50	6.30	0.540
	030001	Medium	1	2	G		104	77.8	64.9	27.7	8.22	3.18	2.46	1.02	6.30	0.780
	030001	Medium	1	2	H		103	71.6	58.9	25.5	9.42	4.14	3.90	1.26	4.38	0.780
Average							103	77.4	58.1	29.0	8.82	5.34	3.63	1.71	5.91	0.840
Std. Dev.							5	4.4	5.3	4.0	2.63	1.97	1.37	0.86	0.83	0.170
Coeff. Var.							4.6	5.6	9.2	13.6	29.8	36.9	37.6	50.0	14.1	20.2
EC50 (µg/ml). Effective concentration at 50% cytotoxicity. (using a specific sigmoidal equation = model).																		123

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Dataset wrap

Jira

showSummary	false
server	Issue Tracker (JIRA)
serverId	85506ce4-3cb3-3d91-85ee-f633aaaf4a45
key	TOBA-279

Rowcaps

Rows 1-12:	Show the test conditions for the set with a short name or SETCD value of ALL. The applicant chose this value to represent test conditions for the entire assay.
Rows 13-20:	Show the test conditions for the set with a short name or SETCD value of CSC-50b. The applicant chose this value to represent plate number 1, well row A, well number 1column 3, with a concentration of 50µg/mLof cigarette smoke condensate as shown in the report table for study 123.
Row 20:	Shows the value for Cell Line (CELLLN) is not applicable because the well is a media-only well, having no NHK cells.
Rows 21-28:	Show the test conditions for the set with a short name or SETCD value of CSC-50. The applicant chose this value to represent plate number 1, well rows B-G, well column 3 and plate number 2, with a , well rows A-H, well column 3. These all have the concentration of 50µg/mLof cigarette smoke condensate as shown in the report table for study 123.
Rows 29-36:	Show the test conditions for the set with a short name or SETCD value of SLS-110. The applicant chose this value to represent both wells numbered 7 in plate number 1, well rows B-G, well column 10, and plate number 2, with a , well rows A-H, well column 10. These all have the concentration of 110µg/mLof sodium laurel sulfate as shown in the report table for study 123.

Dataset2

Row	STUDYID	GNTXAID	DOMAIN	SETCD	SET	TXSEQ	TXPARMCD	TXPARM	TXVAL
1	123	NRU	TX	ALL	ALL_ASSAY	1	IVTDMIN	In vitro Treatment Duration Minimum	47.5
2	123	NRU	TX	ALL	ALL_ASSAY	2	IVTDTRG	In vitro Treatment Duration Target	48
3	123	NRU	TX	ALL	ALL_ASSAY	3	IVTDMAX	In vitro Treatment Duration Maximum	48.5
4	123	NRU	TX	ALL	ALL_ASSAY	4	IVTDU	In vitro Treatment Duration Unit	HOURS
5	123	NRU	TX	ALL	ALL_ASSAY	5	INCBTMP	Incubation Temperature	37
6	123	NRU	TX	ALL	ALL_ASSAY	6	INCBTMPU	Incubation Temperature Unit	C
7	123	NRU	TX	ALL	ALL_ASSAY	7	SWAVENM	Spectrophotometer Wavelength	540
8	123	NRU	TX	ALL	ALL_ASSAY	8	ATMRHP	Atmospheric Relative Humidity Percent	90
9	123	NRU	TX	ALL	ALL_ASSAY	9	ATMCO2P	Atmospheric CO2 Percent	5
10	123	NRU	TX	ALL	ALL_ASSAY	10	SPDEVID	applicant defined device identifier	PUFFMASTER3k
11	123	NRU	TX	ALL	ALL_ASSAY	11	SMKRGM	Smoking Regimen	MEDIUM INTENSITY REGIMEN
12	123	NRU	TX	ALL	ALL_ASSAY	12	SPTOBID	Applicant-defined tobacco identifier	CIG01a
13	123	NRU	TX	CSC-50b	CSC-50-blank	13	PSETCD	Parent Set Code	ALL
14	123	NRU	TX	CSC-50b	CSC-50-blank	14	EXPTYP	Exposure Type	Air Liquid Interface
15	123	NRU	TX	CSC-50b	CSC-50-blank	15	SAMTYP	Sample Type	Whole Smoke Conditioned Media
16	123	NRU	TX	CSC-50b	CSC-50-blank	16	ITVNAM	Name of the Intervention Article	Tobacco ProdA
17	123	NRU	TX	CSC-50b	CSC-50-blank	17	ITVTYPE	Intervention Article Type	PRODUCT
18	123	NRU	TX	CSC-50b	CSC-50-blank	18	ITVCONC	Intervention Article Concentration	50
19	123	NRU	TX	CSC-50b	CSC-50-blank	19	ITVCONCU	Intervention Article Concentration Unit	ug/ml
20	123	NRU	TX	CSC-50b	CSC-50-blank	20	CELLLN	Cell Line	NOT APPLICABLE
21	123	NRU	TX	CSC-50	CSC-50	21	PSETCD	Parent Set Code	ALL
22	123	NRU	TX	CSC-50	CSC-50	22	EXPTYP	Exposure Type	Air Liquid Interface
23	123	NRU	TX	CSC-50	CSC-50	23	SAMTYP	Sample Type	Whole Smoke Conditioned Media
24	123	NRU	TX	CSC-50	CSC-50	24	ITVNAM	Intervention Article Name	Tobacco ProdA
25	123	NRU	TX	CSC-50	CSC-50	25	ITVTYPE	Intervention Article Type	PRODUCT
26	123	NRU	TX	CSC-50	CSC-50	26	ITVCONC	Intervention Article Concentration	50
27	123	NRU	TX	CSC-50	CSC-50	27	ITVCONCU	Intervention Article Concentration Unit	ug/ml
28	123	NRU	TX	CSC-50	CSC-50	28	CELLLN	Cell Line	NHK
29	123	NRU	TX	SLS-110	SLS-110	29	PSETCD	Parent Set Code	ALL
30	123	NRU	TX	SLS-110	SLS-110	30	EXPTYP	Exposure Type	Air Liquid Interface
31	123	NRU	TX	SLS-110	SLS-110	31	SAMTYP	Sample Type	Liquid
32	123	NRU	TX	SLS-110	SLS-110	32	ITVNAM	Intervention Article Name	Sodium Laurel Sulfate
33	123	NRU	TX	SLS-110	SLS-110	33	ITVTYPE	Intervention Article Type	POSITIVE CONTROL
34	123	NRU	TX	SLS-110	SLS-110	34	ITVCONC	Intervention Article Concentration	110
35	123	NRU	TX	SLS-110	SLS-110	35	ITVCONCU	Intervention Article Concentration Unit	ug/ml
36	123	NRU	TX	SLS-110	SLS-110	36	CELLLN	Cell Line	NHK

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