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This example shows trial design and results data for in vitro Neutral Red Uptake Cytotoxicity Assay, Relative Absorbance.

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Note that Variable Names and Domain abbreviations may change (have not been checked against existing and are not vetted)

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titleStudy 123, Report Table

The following shows the tabulated data as it is represented in a report for this study.  This report includes summary statistics (e.g. Average, Standard Deviation, Coeff. Var.); however, statistics are not included in the SEND standard and will not be represented in the SEND domains example. 

Neutral Red Cytotoxicity Assay Results (Relative Absorbance Data)



Run-Port Number

Sample ID

Smoke Fraction

Replicate Number

Plate Number

Well Number


Relative Assay Plate Absorbance Readings




Cigarette Smoke Condensate (µg/mL)

SLS (µg/mL)


EC50 (µg/ml)

0

10

50

75

100

120

140

160

200

110

200

0


1-1

030001

A

1

1

1

0.046

0.046*0.039*0.012*0.030*0.003*0.057*0.032*0.003*0.050*0.044*

0.040


1-1

030001

A

1

1

2

0.048

107

77.7

65.7

30.2

15.6

9.79

5.41

2.49

8.09

0.791

0.014


1-1

030001

A

1

1

3

0.005

98.6

77.4

57.7

35.8

8.09

7.36

3.22

0.791

6.38

0.061

0.050


1-1

030001

A

1

1

4

0.049

94.9

81.5

38.2

31.2

7.60

5.17

3.22

0.791

5.17

0.304

0.017


1-1

030001

A

1

1

5

0.047

111

78.1

64.5

32.9

8.82

5.90

2.74

1.28

10.3

0.547

0.011


1-1

030001

A

1

1

6

0.032

96.6

76.2

48.0

26.8

7.84

4.20

2.74

1.52

8.09

4.93

0.022


1-1

030001

A

1

1

7

0.044

105

77.9

58.2

28.3

8.09

4.68

1.76

0.547

8.57

1.76

0.056


1-1

030001

A

1

1

8

0.056

0.013*0.040*0.008*0.013*0.002*0.029*0.018*0.006*0.016*0.024*

0.046


Average


101

78.2

54.5

29.9

9.70

6.17

3.37

1.19

8.94

1.22



Std. Dev.


6

2.9

9.4

3.6

2.95

2.19

1.12

0.72

2.72

1.58



Coeff. Var.


5.8

3.6

17.2

11.9

30.4

35.5

33.1

61.0

30.5

129.9



*non-treated control wells














1-1

030001

A

1

2

1


93.4

74.0

56.0

35.1

11.8

8.70

5.10

1.98

5.34

1.02



1-1

030001

A

1

2

2


102

75.2

53.3

35.1

11.8

7.74

5.58

3.66

5.34

1.02



1-1

030001

A

1

2

3


107

79.0

55.0

26.5

10.1

6.06

3.66

1.74

7.02

0.780



1-1

030001

A

1

2

4


104

80.2

55.3

29.8

8.94

3.90

3.66

1.50

6.30

0.780



1-1

030001

A

1

2

5


101

75.7

54.1

26.7

4.86

4.38

1.26

1.02

6.30

1.02



1-1

030001

A

1

2

6


110

85.7

67.7

26.0

5.34

4.62

3.42

1.50

6.30

0.540



1-1

030001

A

1

2

7


104

77.8

64.9

27.7

8.22

3.18

2.46

1.02

6.30

0.780



1-1

030001

A

1

2

8


103

71.6

58.9

25.5

9.42

4.14

3.90

1.26

4.38

0.780



Average


103

77.4

58.1

29.0

8.82

5.34

3.63

1.71

5.91

0.840



Std. Dev.


5

4.4

5.3

4.0

2.63

1.97

1.37

0.86

0.83

0.170



Coeff. Var.


4.6

5.6

9.2

13.6

29.8

36.9

37.6

50.0

14.1

20.2



EC50 (µg/ml). Effective concentration at 50% cytotoxicity.  (using a specific sigmoidal equation = model). (could also be EC20 or EC30, EC70, etc.)













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